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Literature summary for 2.7.1.107 extracted from

  • Takahashi, D.; Suzuki, K.; Sakamoto, T.; Iwamoto, T.; Murata, T.; Sakane, F.
    Crystal structure and calcium-induced conformational changes of diacylglycerol kinase alpha EF-hand domains (2019), Protein Sci., 28, 694-706 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.107

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
Ca2+-bound EF-hand domain of isoform DGKalpha, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.0, 1.0 M succinic acid, and 1% (w/v) PEG monomethyl ether 2000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
E134Q the mutant shows impaired Ca2+ binding Homo sapiens
E179Q the mutant is not able to bind Ca2+ Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme is positively regulated by Ca2+ binding to its N-terminal EF-hand domains. Cooperative binding of two Ca2+ ions dissociates the dimer into a well-folded monomer, which resists to proteolysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol Homo sapiens
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P23743
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, Superdex 75 gel filtration and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol
-
 Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 10000, isoform DGKalpha-EF domain, SDS-PAGE Homo sapiens
monomer 1 * 10600, isoform DGKalpha-EF domain, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
DGKalpha isoform Homo sapiens
diacylglycerol kinase alpha isoform Homo sapiens