Cloned (Comment) | Organism |
---|---|
expressed in Sf21 insect cells | Homo sapiens |
General Stability | Organism |
---|---|
the recombinant isoform DGKepsilon and a truncated version lacking the first 40 residues lose activity upon being stored, particularly upon freezing and thawing, which is minimized by the addition of glycerol. | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | Homo sapiens | specific substrate of isoform DGKepsilon | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P52429 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, purified isoform DGKepsilon and DGKepsilonDELTA40, 14 weeks, no significant loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | specific substrate of isoform DGKepsilon | Homo sapiens | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, His6-tagged enzyme lacking the first 40 residues, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DGKepsilon | isoform | Homo sapiens |
diacylglycerol kinase epsilon | isoform | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
after 6.5 h at 4°C, isoform DGKepsilon and DGKepsilonDELTA40 retain 19.1% and 15.8% of their initial activities, respectively, and 48.2% and 38.1% when stored in 50% glycerol, respectively | Homo sapiens |
25 | - |
recombinant purified isoform DGKepsilon and DGKepsilonDELTA40 are largely inactivated after being stored at room temperature for 7.5 h. After incubation at room temperature for 2 h, the proportions of activity that isoforms DGKepsilon and DGKepsilonDELTA40 retain are 35.2 and 50.3%, respectively | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | dysregulation of isoform DGKepsilon perturbs lipid signalling and biosynthesis, which has been linked to epilepsy, Huntington`s disease, and heart disease. Recessive loss-of-function mutations in the isoform DGKepsilon gene cause atypical haemolytic uremic syndrome | Homo sapiens |