Literature summary for 2.7.1.107 extracted from

Jennings, W.; Doshi, S.; Hota, P.K.; Prodeus, A.; Black, S.; Epand, R.M.
Expression, purification, and properties of a human arachidonoyl-specific isoform of diacylglycerol kinase (2017), Biochemistry, 56, 1337-1347 .

Search for more literature for 2.7.1.107

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf21 insect cells	Homo sapiens

General Stability

General Stability	Organism
the recombinant isoform DGKepsilon and a truncated version lacking the first 40 residues lose activity upon being stored, particularly upon freezing and thawing, which is minimized by the addition of glycerol.	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol	Homo sapiens	specific substrate of isoform DGKepsilon	ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P52429	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Homo sapiens

Storage Stability

Storage Stability	Organism
-80°C, purified isoform DGKepsilon and DGKepsilonDELTA40, 14 weeks, no significant loss of activity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol	specific substrate of isoform DGKepsilon	Homo sapiens	ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 64000, His6-tagged enzyme lacking the first 40 residues, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
DGKepsilon	isoform	Homo sapiens
diacylglycerol kinase epsilon	isoform	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	after 6.5 h at 4°C, isoform DGKepsilon and DGKepsilonDELTA40 retain 19.1% and 15.8% of their initial activities, respectively, and 48.2% and 38.1% when stored in 50% glycerol, respectively	Homo sapiens
25	-	recombinant purified isoform DGKepsilon and DGKepsilonDELTA40 are largely inactivated after being stored at room temperature for 7.5 h. After incubation at room temperature for 2 h, the proportions of activity that isoforms DGKepsilon and DGKepsilonDELTA40 retain are 35.2 and 50.3%, respectively	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	dysregulation of isoform DGKepsilon perturbs lipid signalling and biosynthesis, which has been linked to epilepsy, Huntington`s disease, and heart disease. Recessive loss-of-function mutations in the isoform DGKepsilon gene cause atypical haemolytic uremic syndrome	Homo sapiens

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Release 2023.1 (January 2023)