Cloned (Comment) | Organism |
---|---|
GFP-tagged DGKh and DGKg co-expressed in human neuroblastoma cells SH-SY5Y, expression of GST-fusion isozyme DGKalpha and DGKgamma in Escherichia coli strain DH5alpha, expression of GST-fusion isozyme DGKbeta in Spodoptera frugiperda Sf9 cells via baculovirus infection system, expression of GST-fusion isozymes DGKalpha, DGKbeta, and DGKgamma in COS-7 cells | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isozyme DGKgamma in hippocampal pyriamidal cells and proximal dendrites | Rattus norvegicus | 5829 | - |
additional information | no isozyme DGKgamma in the nucleus, subcellular localization of isozymes in neurons | Rattus norvegicus | - |
- |
plasma membrane | isozyme DGKbeta predominantly, in hippocampal pyriamidal neurons including perikarya and the peripheral dendrites, dendrit cell body | Rattus norvegicus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, isozymes DGKalpha, DGKbeta, and DGKgamma | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | Rattus norvegicus | termination of diacylglycerol signaling, isozymes DGKalpha, DGKbeta, and DGKgamma play a pivotal role in development and metabolism of brain | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
neuron-specific isozymes DGKbeta and DGKgamma | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion isozymes DGKalpha, DGKgamma, and DGKbeta from Escherichia coli, and Sf9 cells, respectively, and from COS-7 cells, by glutathione affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | post-natal, developing, neuron-specific isozymes DGKbeta and DGKgamma, and isozyme DGKalpha, isozymes alpha and gamma appear at birth and then decline, while isozyme beta appears about 14 days after birth and reaches a maximum at day 28, overview | Rattus norvegicus | - |
hippocampus | pyramidal cells, isozymes DGKbeta and DGKgamma | Rattus norvegicus | - |
medial geniculate nucleus | isozyme DGKgamma, day 3-14 after birth | Rattus norvegicus | - |
neuron | neuron-specific isozymes DGKbeta and DGKgamma having a spatio-temporally different function in the neuron | Rattus norvegicus | - |
Purkinje cell | delay of isozyme DGKgamma | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacylglycerol | - |
Rattus norvegicus | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
ATP + 1,2-diacylglycerol | termination of diacylglycerol signaling, isozymes DGKalpha, DGKbeta, and DGKgamma play a pivotal role in development and metabolism of brain | Rattus norvegicus | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 88000-90000, isozymes DGKalpha, DGKbeta, and DGKgamma, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
DGK | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |