Activating Compound | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | enhances activity | Sus scrofa | |
lysophosphatidylcholine | activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin | Sus scrofa | |
phosphatidylcholine | activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin | Sus scrofa | |
phosphatidylethanolamine | activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin | Sus scrofa | |
phosphatidylserine | activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin | Sus scrofa | |
Phospholipid | enhances activity. Activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin | Sus scrofa | |
sphingomyelin | activation of phospholipids in the order of decreasing efficiency: phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphatidate | moderate | Sus scrofa | |
phosphatidylglycerol | inhibits phosphatidylcholine-dependent kinase activity | Sus scrofa | |
phosphatidylinositol | 2.5 mol% results in 50% inhibition of the phosphatidylcholine-dependent kinase activity | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ATP | pH 7.4, 30°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
gel filtration | Sus scrofa |
78000 | - |
1 * 78000, SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, enzyme concentrated by polyethyleneglycol 6000, stable for 1 week | Sus scrofa |
4°C, purified enzyme, 50% loss of activity after 3 days | Sus scrofa |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 78000, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
phosphatidylcholine-dependent activity | Sus scrofa |
8 | - |
broad, deoxycholate-dependent or phosphatidylcholine-dependent activity | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, phosphatidylcholine-dependent activity | Sus scrofa |