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Literature summary for 2.7.1.107 extracted from

  • Walsh, J.P.; Bell, R.M.
    sn-1,2-Diacylglycerol kinase of Escherichia coli. Mixed micellar analysis of the phospholipid cofactor requirement and divalent cation dependence (1986), J. Biol. Chem., 261, 6239-6247.
    View publication on PubMed
Search for more literature for 2.7.1.107

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin half-maximal activation by 1 mol% Rattus norvegicus
Phospholipid activation by phospholipid is not stereospecific and is mimicked partially by fatty acids Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
dioctanoylglycerol above 3.4 mol% Rattus norvegicus
dioleoylglycerol above 3.4 mol% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values are 0.92 mol% for dioleoylglycerol and 3.6 mol% for dioctanoylglycerol Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ when Mg2+ is excluded from the assay, Cd2+ supports activity to lesser extent Rattus norvegicus
Co2+ when Mg2+ is excluded from the assay, Co2+ supports activity to lesser extent Rattus norvegicus
Mg2+ a second Mg2+ in addition to MgATP is required for activity Rattus norvegicus
Mn2+ when Mg2+ is excluded from the assay, Mn2+ supports activity to lesser extent Rattus norvegicus
Zn2+ when Mg2+ is excluded from the assay, Zn2+ supports activity to lesser extent Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-dioctanoylglycerol
-
 Rattus norvegicus ADP + 1,2-dioctanoylglycerol 3-phosphate
-
 ?
ATP + sn-1,2-dioleoylglycerol
-
 Rattus norvegicus ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
 ?
additional information the enzyme is active in mixed micelles containing octyl glucoside and dioleoylglycerol Rattus norvegicus ?
-
 ?