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Literature summary for 2.7.1.107 extracted from

  • Kato, M.; Takenawa, T.
    Purification and characterization of membrane-bound and cytosolic forms of diacylglycerol kinase from rat brain (1990), J. Biol. Chem., 265, 794-800.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
detergent no activity in absence of detergent Rattus norvegicus
phosphatidic acid good activator of DGK IV, no effect on DGK I activity Rattus norvegicus
phosphatidylcholine moderate enhancement of DGK IV, no effect on DGK I activity Rattus norvegicus
phosphatidylinositol enhances activity of DGK I and DGK IV Rattus norvegicus
phosphatidylserine enhances activity of DGK I and DGK IV Rattus norvegicus
sodium cholate enhances activity of DGK I and DGK IV Rattus norvegicus
sodium deoxycholate enhances activity of DGK I and DGK IV Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
adenosine 0.5 mM, 5% inhibition of DGK I and 28% inhibition of DGK IV Rattus norvegicus
ADP 0.5 mM, 77% inhibition of DGK I and 66% inhibition of DGK IV Rattus norvegicus
AMP 0.5 mM, 18% inhibition of DGK I and 14% inhibition of DGK IV Rattus norvegicus
ATPgammaS 0.5 mM, 93% inhibition of DGK I and 71% inhibition of DGK IV Rattus norvegicus
cAMP 0.5 mM, 20% inhibition of DGK I and 15% inhibition of DGK IV Rattus norvegicus
CDP 0.5 mM, 43% inhibition of DGK I and 28% inhibition of DGK IV Rattus norvegicus
CTP 0.5 mM, 42% inhibition of DGK I and 9% inhibition of DGK IV Rattus norvegicus
GDP 0.5 mM, 74% inhibition of DGK I and 72% inhibition of DGK IV Rattus norvegicus
GTP 0.5 mM, 74% inhibition of DGK I and 56% inhibition of DGK IV Rattus norvegicus
GTPgammaS 0.5 mM, 37% inhibition of DGK I and 32% inhibition of DGK IV Rattus norvegicus
quercetin 0.1 mM, 41% inhibition of DGK I and 15% inhibition of DGK IV Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
1-stearoyl-2-arachidonoyl-sn-glycerol pH 7.4, 30°C, enzyme form DGK I Rattus norvegicus
0.09
-
1,2-diarachidonoyl-glycerol pH 7.4, 30°C, enzyme form DGK I Rattus norvegicus
0.09
-
1-stearoyl-2-arachidonoyl-sn-glycerol pH 7.4, 30°C, enzyme form DGK IV Rattus norvegicus
0.1
-
sn-1,2-dioleoylglycerol pH 7.4, 30°C, enzyme form DGK IV Rattus norvegicus
0.125
-
sn-1,2-dioleoylglycerol pH 7.4, 30°C, enzyme form DGK I Rattus norvegicus
0.125
-
ATP pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK I Rattus norvegicus
0.14
-
1,2-diarachidonoyl-glycerol pH 7.4, 30°C, enzyme form DGK IV Rattus norvegicus
0.25
-
ATP pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK IV Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol DGK I Rattus norvegicus 5829
-
membrane bound to Rattus norvegicus 16020
-
membrane DGK IV Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ DGK I: good activator, DGK IV: activation with 1 mM CaCl2 is comparable to that obtained on presence of MgCl2 Rattus norvegicus
Mg2+ DGK I: maximal activation at 20 mM. DGK IV: maximal activation at 5 mM Rattus norvegicus
Mn2+ DGK I: activation is not so great as with MgCl2. Maximal stimulation at 0.1 mM. DGK IV: activation is 10fold less than with MgCl2 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DGK I and DGK IV Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
heart low activity Rattus norvegicus
-
kidney low activity Rattus norvegicus
-
liver low activity Rattus norvegicus
-
lung
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
thymus
-
Rattus norvegicus
-
uterus low activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.187
-
enzyme form DGK IV Rattus norvegicus
11.5
-
enzyne form DGK I Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diarachidonoyl-glycerol
-
Rattus norvegicus ADP + 1,2-diarachidonoyl-glycerol 3-phosphate
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
-
Rattus norvegicus ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
?
ATP + sn-1,2-dioleoylglycerol
-
Rattus norvegicus ADP + sn-1,2-dioleoylglycerol 3-phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
DGK I and DGK IV Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity Rattus norvegicus
6.8 9 pH 6.5: about 85% of maximal activity pH 9.0: about 50% of maximal activity, enzyme form DGK I Rattus norvegicus