Activating Compound | Comment | Organism | Structure |
---|---|---|---|
detergent | no activity in absence of detergent | Rattus norvegicus | |
phosphatidic acid | good activator of DGK IV, no effect on DGK I activity | Rattus norvegicus | |
phosphatidylcholine | moderate enhancement of DGK IV, no effect on DGK I activity | Rattus norvegicus | |
phosphatidylinositol | enhances activity of DGK I and DGK IV | Rattus norvegicus | |
phosphatidylserine | enhances activity of DGK I and DGK IV | Rattus norvegicus | |
sodium cholate | enhances activity of DGK I and DGK IV | Rattus norvegicus | |
sodium deoxycholate | enhances activity of DGK I and DGK IV | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | 0.5 mM, 5% inhibition of DGK I and 28% inhibition of DGK IV | Rattus norvegicus | |
ADP | 0.5 mM, 77% inhibition of DGK I and 66% inhibition of DGK IV | Rattus norvegicus | |
AMP | 0.5 mM, 18% inhibition of DGK I and 14% inhibition of DGK IV | Rattus norvegicus | |
ATPgammaS | 0.5 mM, 93% inhibition of DGK I and 71% inhibition of DGK IV | Rattus norvegicus | |
cAMP | 0.5 mM, 20% inhibition of DGK I and 15% inhibition of DGK IV | Rattus norvegicus | |
CDP | 0.5 mM, 43% inhibition of DGK I and 28% inhibition of DGK IV | Rattus norvegicus | |
CTP | 0.5 mM, 42% inhibition of DGK I and 9% inhibition of DGK IV | Rattus norvegicus | |
GDP | 0.5 mM, 74% inhibition of DGK I and 72% inhibition of DGK IV | Rattus norvegicus | |
GTP | 0.5 mM, 74% inhibition of DGK I and 56% inhibition of DGK IV | Rattus norvegicus | |
GTPgammaS | 0.5 mM, 37% inhibition of DGK I and 32% inhibition of DGK IV | Rattus norvegicus | |
quercetin | 0.1 mM, 41% inhibition of DGK I and 15% inhibition of DGK IV | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
1-stearoyl-2-arachidonoyl-sn-glycerol | pH 7.4, 30°C, enzyme form DGK I | Rattus norvegicus | |
0.09 | - |
1,2-diarachidonoyl-glycerol | pH 7.4, 30°C, enzyme form DGK I | Rattus norvegicus | |
0.09 | - |
1-stearoyl-2-arachidonoyl-sn-glycerol | pH 7.4, 30°C, enzyme form DGK IV | Rattus norvegicus | |
0.1 | - |
sn-1,2-dioleoylglycerol | pH 7.4, 30°C, enzyme form DGK IV | Rattus norvegicus | |
0.125 | - |
sn-1,2-dioleoylglycerol | pH 7.4, 30°C, enzyme form DGK I | Rattus norvegicus | |
0.125 | - |
ATP | pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK I | Rattus norvegicus | |
0.14 | - |
1,2-diarachidonoyl-glycerol | pH 7.4, 30°C, enzyme form DGK IV | Rattus norvegicus | |
0.25 | - |
ATP | pH 7.4, 30°C, in presence of 1,2-dioleoylglycerol, enzyme form DGK IV | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | DGK I | Rattus norvegicus | 5829 | - |
membrane | bound to | Rattus norvegicus | 16020 | - |
membrane | DGK IV | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | DGK I: good activator, DGK IV: activation with 1 mM CaCl2 is comparable to that obtained on presence of MgCl2 | Rattus norvegicus | |
Mg2+ | DGK I: maximal activation at 20 mM. DGK IV: maximal activation at 5 mM | Rattus norvegicus | |
Mn2+ | DGK I: activation is not so great as with MgCl2. Maximal stimulation at 0.1 mM. DGK IV: activation is 10fold less than with MgCl2 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DGK I and DGK IV | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
heart | low activity | Rattus norvegicus | - |
kidney | low activity | Rattus norvegicus | - |
liver | low activity | Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
thymus | - |
Rattus norvegicus | - |
uterus | low activity | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.187 | - |
enzyme form DGK IV | Rattus norvegicus |
11.5 | - |
enzyne form DGK I | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diarachidonoyl-glycerol | - |
Rattus norvegicus | ADP + 1,2-diarachidonoyl-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | - |
Rattus norvegicus | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + sn-1,2-dioleoylglycerol | - |
Rattus norvegicus | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
DGK I and DGK IV | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity | Rattus norvegicus |
6.8 | 9 | pH 6.5: about 85% of maximal activity pH 9.0: about 50% of maximal activity, enzyme form DGK I | Rattus norvegicus |