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Literature summary for 2.7.1.105 extracted from

  • Parducci, R.E.; Cabrera, R.; Baez, M.; Guixe, V.
    Evidence for a catalytic Mg2+ ion and effect of phosphate on the activity of Escherichia coli phosphofructokinase-2: regulatory properties of a ribokinase (2006), Biochemistry, 45, 9291-9299.
    View publication on PubMed
Search for more literature for 2.7.1.105

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E190Q mutant presents a 50fold decrease in the kcat value and a 15fold increment in the apparent Km for MgATP2+. E190Q mutant presents alterations in the inhibition by MgATP2- and phosphate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
MgATP2- E190Q mutant presents alterations in the inhibition by MgATP2- and phosphate Escherichia coli
phosphate inhibits wild-type and mutant enzym E190Q. E190 contributs to the mechanism of phosphate inhibition in Pfk-2. E190Q mutant presents alterations in the inhibition by MgATP2- and phosphate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
 D-fructose 6-phosphate mutant enzyme E190Q, 30 mM Mg2+ Escherichia coli
0.01
-
 D-fructose 6-phosphate mutant enzyme E190Q, 1 mM Mg2+ Escherichia coli
0.02
-
 MgATP2- wild-type enzyme, 1 mM Mg2+ Escherichia coli
0.022
-
 MgATP2- wild-type enzyme, 30 mM Mg2+ Escherichia coli
0.028
-
 D-fructose 6-phosphate wild-type enzyme, 30 mM Mg2+ Escherichia coli
0.1
-
 D-fructose 6-phosphate wild-type enzyme, 1 mM Mg2+ Escherichia coli
0.149
-
 MgATP2- mutant enzyme E190Q, 1 mM Mg2+ Escherichia coli
0.32
-
 MgATP2- mutant enzyme E190Q, 30 mM Mg2+ Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement of a Mg2+ ion, besides the one present in the metal-nucleotide complex, for catalysis in the wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Escherichia coli ADP + D-fructose 2,6-bisphosphate
-
 ?
MgATP2- + beta-D-fructose 6-phosphate
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
Pfk-2
-
 Escherichia coli
phosphofructokinase-2
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 D-fructose 6-phosphate mutant enzyme E190Q, 1 mM Mg2+ Escherichia coli
0.14
-
 MgATP2- mutant enzyme E190Q, 1 mM Mg2+ Escherichia coli
1.43
-
 D-fructose 6-phosphate mutant enzyme E190Q, 30 mM Mg2+ Escherichia coli
1.43
-
 MgATP2- mutant enzyme E190Q, 30 mM Mg2+ Escherichia coli
53
-
 D-fructose 6-phosphate wild-type enzyme, 1 mM Mg2+ Escherichia coli
53
-
 MgATP2- wild-type enzyme, 1 mM Mg2+ Escherichia coli
53
-
 D-fructose 6-phosphate wild-type enzyme, 30 mM Mg2+ Escherichia coli
53
-
 MgATP2- wild-type enzyme, 30 mM Mg2+ Escherichia coli