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Literature summary for 2.7.1.105 extracted from
- Kinetic properties of bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from spinach leaves (2002), Eur. J. Biochem., 269, 1267-1277.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Spodoptera frugiperda cells infected with recombinant baculovirus and Escherichia coli | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | - |
Spinacia oleracea |  |
| dihydroxyacetone phosphate | - |
Spinacia oleracea | |
| diphosphate | not phosphate | Spinacia oleracea | |
| glycerate 2-phosphate | - |
Spinacia oleracea | |
| phosphoenolpyruvate | - |
Spinacia oleracea | |
| sn-glycerol 3-phosphate | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.32 | - |
ATP | pH 7.8, 25°C, effect of phosphate on Km | Spinacia oleracea | |
| 1.4 | - |
beta-D-fructose 6-phosphate | pH 7.8, 25°C, effect of phosphate on Km | Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | activation | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90800 | - |
4 * 90800, SDS-PAGE | Spinacia oleracea |
| 320000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | Spinacia oleracea | responsible for regulation of fructose 2,6-bisphosphate-concentration | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | O64983 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzymes | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0104 | 0.0159 | - |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | - |
Spinacia oleracea | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
| ATP + beta-D-fructose 6-phosphate | responsible for regulation of fructose 2,6-bisphosphate-concentration | Spinacia oleracea | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 90800, SDS-PAGE | Spinacia oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Spinacia oleracea |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
glycerate 2-phosphate | pH 7.8, 25°C | Spinacia oleracea | |
| 0.045 | - |
phosphoenolpyruvate | pH 7.8, 25°C | Spinacia oleracea | |
| 0.084 | - |
Glycerate 3-phosphate | pH 7.8, 25°C | Spinacia oleracea | |
| 0.106 | - |
diphosphate | pH 7.8, 25°C | Spinacia oleracea | |
| 0.737 | - |
dihydroxyacetone phosphate | pH 7.8, 25°C | Spinacia oleracea | |
| 8.07 | - |
sn-glycerol 3-phosphate | pH 7.8, 25°C | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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