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Literature summary for 2.7.1.105 extracted from

  • Bertrand, L.; Alessi, D.R.; Deprez, J.; Deak, M.; Viaene, E.; Rider, M.H.; Hue, L.
    Heart 6-phosphofructo-2-kinase activation by insulin results from Ser-466 and Ser-483 phosphorylation and requires 3-phosphoinositide-dependent kinase-1, but not protein kinase B (1999), J. Biol. Chem., 274, 30927-30933.
    View publication on PubMed
Search for more literature for 2.7.1.105

Activating Compound

Activating Compound Comment Organism Structure
Insulin activation of transfected heart enzyme in human embryonic kidney 293 cells by inducing the phosphorylation of Ser466 and Ser483, activation is mediated by 3-phosphoinositide-dependent kinase-1, protein kinase B is in vivo not essential, activation is sensitive to LY294002 and wortmannin and insensitive to rapaycin and PD98059 Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme, S466E and S466E/S483E mutants are expressed in Escherichia coli BL21(DE3)pLysE and S483E mutant is expressed in BL21(DE3)pLysS, human embryonic kidney 293 cells are transiently transfected with vectors expressing wild-type heart enzyme Bos taurus

Protein Variants

Protein Variants Comment Organism
S466E mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme in vitro Bos taurus
S466E/S483E the double mutant is not phosphorylated by protein kinase B, mutation decreases Km of beta-D-fructose 6-phosphate in vitro Bos taurus
S483E mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme and mutation decreases citrate inhibition in vitro Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
citrate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information comparison of Km of wild-type and mutant enzyme Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzyme Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Bos taurus