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Literature summary for 2.7.1.105 extracted from

  • Tominaga, N.; Tsujikawa, T.; Minami, Y.; Wu, R.F.; Watanabe, F.; Sakakibara, R.; Uyeda, K.
    Effect of replacement of the amino and the carboxyl termini of rat testis fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase with those of the liver and heart isozymes (1997), Arch. Biochem. Biophys., 347, 275-281.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Rattus norvegicus
-
Bos taurus

Protein Variants

Protein Variants Comment Organism
additional information mutant enzymes of rat testis are constructed, in which its terminal peptides are replaced with those of the liver or the heart enzyme Rattus norvegicus
additional information mutant enzymes of rat testis are constructed, in which its terminal peptides are replaced with those of the liver or the heart enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
phosphoenolpyruvate
-
Bos taurus
phosphoenolpyruvate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
beta-D-fructose 6-phosphate pH 7.5, 25°C, heart Bos taurus
0.023
-
beta-D-fructose 6-phosphate comparison of Km of wild-type, mutant and mutant phosphorylated enzyme Rattus norvegicus
0.023
-
beta-D-fructose 6-phosphate comparison of Km of wild-type, mutant and mutant phosphorylated enzyme Bos taurus
0.025
-
beta-D-fructose 6-phosphate pH 7.5, 30°C, liver, comparison of Km of wild-type, mutant and mutant phosphorylated enzyme Rattus norvegicus
0.04
-
beta-D-fructose 6-phosphate pH 7.5, 30°C, wild-type enzyme, testis Rattus norvegicus
0.061
-
ATP pH 7.5, 30°C, liver Rattus norvegicus
0.083
-
ATP pH 7.5, 25°C, heart Bos taurus
0.1
-
ATP
-
Bos taurus
0.1
-
ATP pH 7.5, 30°C, testis Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme Rattus norvegicus
mutant enzyme Bos taurus

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Rattus norvegicus
-
heart
-
Bos taurus
-
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-
testis
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
r
ATP + beta-D-fructose 6-phosphate
-
Bos taurus ADP + beta-D-fructose 2,6-bisphosphate
-
r
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
?
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Bos taurus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Rattus norvegicus
25
-
assay at Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information comparison of kcat/Km values of wild-type and mutant enzymes Rattus norvegicus
additional information
-
additional information comparison of kcat/Km values of wild-type and mutant enzymes Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information comparison of Ki of wild type and mutant enzymes Rattus norvegicus
0.014
-
phosphoenolpyruvate pH 7.5, 30°C, testis Rattus norvegicus
0.08
-
phosphoenolpyruvate pH 7.5, 30°C, liver Rattus norvegicus
0.1
-
phosphoenolpyruvate pH 7.5, 30°C, heart Bos taurus