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Literature summary for 2.7.1.105 extracted from

  • Mizuguchi, H.; Cook, P.F.; Hasemann, C.A.; Uyeda, K.
    Chemical mechanism of the fructose-6-phosphate,2-kinase reaction from the pH dependence of kinetic parameters of site-directed mutants of active site basic residues (1997), Biochemistry, 36, 8775-8784.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
K172A increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
K172E increase in Km of MgATP2-, increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
K172H increase in Km of MgATP2- Rattus norvegicus
K172R increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
K51A increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
K51H increase in Km of MgATP2-, increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
additional information ATP binding and the effect of pH on the kinetics are characterized Rattus norvegicus
R136K increase in Km of MgATP2- Rattus norvegicus
R136L increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
R193H increase in Km of MgATP2-, increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
R193L increase in Km of beta-D-fructose 6-phosphate Rattus norvegicus
R78H increase in Km of MgATP2- Rattus norvegicus
R78L increase in Km of MgATP2- Rattus norvegicus
R79H increase in Km of MgATP2- Rattus norvegicus
R79L increase in Km of MgATP2- Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Rattus norvegicus
0.04
-
beta-D-fructose 6-phosphate pH 7.5, 30°C, wild-type enzyme, testis Rattus norvegicus
0.1
-
ATP pH 7.5, 30°C, testis Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ K51 is essential for binding of Mg(mantATP) Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
r
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information comparison of kcat/Km values of wild-type and mutant enzymes Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
comparison of pH-profiles of wild-type and mutant enzymes Rattus norvegicus
7.3 8.4 wild-type enzyme Rattus norvegicus