Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K172A | increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
K172E | increase in Km of MgATP2-, increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
K172H | increase in Km of MgATP2- | Rattus norvegicus |
K172R | increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
K51A | increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
K51H | increase in Km of MgATP2-, increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
additional information | ATP binding and the effect of pH on the kinetics are characterized | Rattus norvegicus |
R136K | increase in Km of MgATP2- | Rattus norvegicus |
R136L | increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
R193H | increase in Km of MgATP2-, increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
R193L | increase in Km of beta-D-fructose 6-phosphate | Rattus norvegicus |
R78H | increase in Km of MgATP2- | Rattus norvegicus |
R78L | increase in Km of MgATP2- | Rattus norvegicus |
R79H | increase in Km of MgATP2- | Rattus norvegicus |
R79L | increase in Km of MgATP2- | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus | |
0.04 | - |
beta-D-fructose 6-phosphate | pH 7.5, 30°C, wild-type enzyme, testis | Rattus norvegicus | |
0.1 | - |
ATP | pH 7.5, 30°C, testis | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | K51 is essential for binding of Mg(mantATP) | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of kcat/Km values of wild-type and mutant enzymes | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of pH-profiles of wild-type and mutant enzymes | Rattus norvegicus |
7.3 | 8.4 | wild-type enzyme | Rattus norvegicus |