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Literature summary for 2.7.1.105 extracted from

  • Lange, A.J.; Li, L.; Vargas, A.M.; Pilkis, S.J.
    Expression of human liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli. Role of N-2 proline in degradation of the protein (1993), J. Biol. Chem., 268, 8078-8084.
    View publication on PubMed
Search for more literature for 2.7.1.105

Cloned(Commentary)

Cloned (Comment) Organism
mutant expressed in Escherichia coli BL21(DL3) Homo sapiens
mutant expressed in Escherichia coli BL21(DL3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
P2R kinetic properties of human liver mutant and rat wild-type enzyme are very similar Homo sapiens
P2R kinetic properties of human liver mutant and rat wild-type enzyme are very similar Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019 0.021 beta-D-fructose 6-phosphate pH 7.8, in presence of phosphate Homo sapiens
0.019 0.021 beta-D-fructose 6-phosphate pH 7.8, in presence of phosphate Rattus norvegicus
0.12
-
 beta-D-fructose 6-phosphate pH 7.8 Rattus norvegicus
0.12 0.36 ATP pH 7.8 Rattus norvegicus
0.4
-
 ATP
-
 Rattus norvegicus
0.4
-
 ATP pH 7.8 Homo sapiens
1 1.1 beta-D-fructose 6-phosphate pH 7.8, absence of phosphate Homo sapiens
1 1.1 beta-D-fructose 6-phosphate pH 7.8, absence of phosphate Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
phosphate
-
 Homo sapiens
phosphate
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 primary structures of human, rat and bovine liver enzyme Homo sapiens
additional information
-
 primary structures of human, rat and bovine liver enzyme Rattus norvegicus
55000
-
 2 * 55000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human enzyme is 95% identical with rat or bovine enzyme
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Homo sapiens
a
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.046
-
-
 Homo sapiens
0.046
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Homo sapiens ?
-
 ?
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Homo sapiens
dimer 2 * 55000, SDS-PAGE Rattus norvegicus