Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.100 extracted from

  • Ferro, A.J.; Marchitto, K.S.
    5-Methylthioribose kinase (Enterobacter aerogenes) (1983), Methods Enzymol., 94, 361-364.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 20 mM, 70% of activity with dithiothreitol Klebsiella aerogenes
dithiothreitol required for activity, maximal activity at 20 mM Klebsiella aerogenes
dithiothreitol can partially be replaced by 20 mM 2-mercaptoethanol or glutathione Klebsiella aerogenes
glutathione 20 mM, 44% of activity with dithiothreitol Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0081
-
5-methylthioribose
-
Klebsiella aerogenes
0.074
-
ATP
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at 5 mM Klebsiella aerogenes
Mg2+ divalent metal ion is strictely required for activity, Mg2+ is most effective Klebsiella aerogenes
Mn2+ 20% of activity with Mg2+ Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-methylthioribose Klebsiella aerogenes may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine ADP + 5-methylthioribose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-200, DEAE-cellulose Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.028
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol, at least 5 mg/ml, stable Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-butylthioribose
-
Klebsiella aerogenes ADP + 5-butylthioribose 1-phosphate
-
?
ATP + 5-ethylthioribose
-
Klebsiella aerogenes ADP + 5-ethylthioribose 1-phosphate
-
?
ATP + 5-isobutylthioribose
-
Klebsiella aerogenes ADP + 5-isobutylthioribose 1-phosphate
-
?
ATP + 5-isopropylthioribose
-
Klebsiella aerogenes ADP + 5-isopropylthioribose 1-phosphate
-
?
ATP + 5-methylthioribose no activity with GTP and UTP Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
?
ATP + 5-methylthioribose may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine Klebsiella aerogenes ADP + 5-methylthioribose 1-phosphate
-
?
ATP + 5-propylthioribose
-
Klebsiella aerogenes ADP + 5-propylthioribose 1-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Klebsiella aerogenes