Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 20 mM, 70% of activity with dithiothreitol | Klebsiella aerogenes | |
dithiothreitol | required for activity, maximal activity at 20 mM | Klebsiella aerogenes | |
dithiothreitol | can partially be replaced by 20 mM 2-mercaptoethanol or glutathione | Klebsiella aerogenes | |
glutathione | 20 mM, 44% of activity with dithiothreitol | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0081 | - |
5-methylthioribose | - |
Klebsiella aerogenes | |
0.074 | - |
ATP | - |
Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 5 mM | Klebsiella aerogenes | |
Mg2+ | divalent metal ion is strictely required for activity, Mg2+ is most effective | Klebsiella aerogenes | |
Mn2+ | 20% of activity with Mg2+ | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-methylthioribose | Klebsiella aerogenes | may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine | ADP + 5-methylthioribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-200, DEAE-cellulose | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.028 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, at least 5 mg/ml, stable | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-butylthioribose | - |
Klebsiella aerogenes | ADP + 5-butylthioribose 1-phosphate | - |
? | |
ATP + 5-ethylthioribose | - |
Klebsiella aerogenes | ADP + 5-ethylthioribose 1-phosphate | - |
? | |
ATP + 5-isobutylthioribose | - |
Klebsiella aerogenes | ADP + 5-isobutylthioribose 1-phosphate | - |
? | |
ATP + 5-isopropylthioribose | - |
Klebsiella aerogenes | ADP + 5-isopropylthioribose 1-phosphate | - |
? | |
ATP + 5-methylthioribose | no activity with GTP and UTP | Klebsiella aerogenes | ADP + 5-methylthioribose 1-phosphate | - |
? | |
ATP + 5-methylthioribose | may be a primary enzyme involved in the recycling of the methylthio group of 5-methylthioribose back into methionine | Klebsiella aerogenes | ADP + 5-methylthioribose 1-phosphate | - |
? | |
ATP + 5-propylthioribose | - |
Klebsiella aerogenes | ADP + 5-propylthioribose 1-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Klebsiella aerogenes |