Literature summary for 2.7.1.1 extracted from

Martinez, J.A.; Larion, M.; Conejo, M.S.; Porter, C.M.; Miller, B.G.
Role of connecting loop I in catalysis and allosteric regulation of human glucokinase (2014), Protein Sci., 23, 915-922.

Search for more literature for 2.7.1.1

Protein Variants

Protein Variants	Comment	Organism
additional information	sequential, single amino acid deletions from the C-terminus of connecting loop I cause systematic decreases in cooperativity. Deleting up to two loop residues leaves the kcat value unchanged, removing three or more residues reduces kcat by 1000fold. In contrast, the glucose K0.5 and KD values are unaffected by shortening the connecting loop by up to six residues. Substituting alanine or glycine for proline66 does not alter cooperativity. Replacing connecting loop I with the corresponding loop sequence from the catalytic domain of the noncooperative isozyme human hexokinase I eliminates cooperativity without impacting the kcat and glucose K0.5 values	Homo sapiens
S64R/E67D/S69T	3fold increase in activity, no change in cooperativity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	ATP	deletion mutant lacking residues 66-71, pH 7.6, 25 °C	Homo sapiens
0.2	-	ATP	deletion mutant lacking residues 68-71, pH 7.6, 25 °C	Homo sapiens
0.29	-	ATP	deletion mutant lacking residue 71, pH 7.6, 25 °C	Homo sapiens
0.3	-	ATP	deletion mutant lacking residues 67-71, pH 7.6, 25 °C	Homo sapiens
0.5	-	ATP	mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C	Homo sapiens
0.5	-	ATP	wild-type, pH 7.6, 25 °C	Homo sapiens
1.2	-	ATP	deletion mutant lacking residues 70-71, pH 7.6, 25 °C	Homo sapiens
2.5	-	D-glucose	deletion mutant lacking residues 68-71, K0.5 value, Hill coefficient 1.2, pH 7.6, 25 °C	Homo sapiens
3.1	-	D-glucose	deletion mutant lacking residue 71, K0.5 value, Hill coefficient 1.5, pH 7.6, 25 °C	Homo sapiens
3.2	-	D-glucose	deletion mutant lacking residues 67-71, K0.5 value, Hill coefficient 1.1, pH 7.6, 25 °C	Homo sapiens
3.3	-	D-glucose	mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C	Homo sapiens
3.6	-	D-glucose	deletion mutant lacking residues 66-71, K0.5 value, Hill coefficient 1.2, pH 7.6, 25 °C	Homo sapiens
6.3	-	D-glucose	wild-type, K0.5 value, Hill coefficient 1.8, pH 7.6, 25 °C	Homo sapiens
10.6	-	D-glucose	deletion mutant lacking residues 70-71, K0.5 value, Hill coefficient 1.3, pH 7.6, 25 °C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35557	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-glucose	-	Homo sapiens	ADP + D-glucose 6-phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.026	-	D-glucose	deletion mutant lacking residues 68-71, pH 7.6, 25 °C	Homo sapiens
0.037	-	D-glucose	deletion mutant lacking residues 66-71, pH 7.6, 25 °C	Homo sapiens
0.042	-	D-glucose	deletion mutant lacking residues 67-71, pH 7.6, 25 °C	Homo sapiens
29	-	D-glucose	deletion mutant lacking residues 70-71, pH 7.6, 25 °C	Homo sapiens
33	-	D-glucose	deletion mutant lacking residue 71, pH 7.6, 25 °C	Homo sapiens
40.1	-	D-glucose	wild-type, pH 7.6, 25 °C	Homo sapiens
56	-	D-glucose	mutant S64R/E67D/S69T, K0.5 value, Hill coefficient 1.7, pH 7.6, 25 °C	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.01	-	D-glucose	deletion mutant lacking residues 66-71, pH 7.6, 25 °C	Homo sapiens
0.011	-	D-glucose	deletion mutant lacking residues 68-71, pH 7.6, 25 °C	Homo sapiens
0.013	-	D-glucose	deletion mutant lacking residues 67-71, pH 7.6, 25 °C	Homo sapiens
2.7	-	D-glucose	deletion mutant lacking residues 70-71, pH 7.6, 25 °C	Homo sapiens
6.3	-	D-glucose	wild-type, pH 7.6, 25 °C	Homo sapiens
11	-	D-glucose	deletion mutant lacking residue 71, pH 7.6, 25 °C	Homo sapiens

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Release 2023.1 (January 2023)