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Literature summary for 2.7.1.1 extracted from

  • Sun, M.; Liao, S.; Zhang, L.; Wu, C.; Qi, N.; Lv, M.; Li, J.; Lin, X.; Zhang, J.; Xie, M.; Zhu, G.; Cai, J.
    Molecular and biochemical characterization of Eimeria tenella hexokinase (2016), Parasitol. Res., 115, 3425-3433.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D217H about 7% of wild-type activity Eimeria tenella
N216K about 2% of wild-type activity Eimeria tenella
S159P complete loss of activity Eimeria tenella

Inhibitors

Inhibitors Comment Organism Structure
mangiferin
-
Eimeria tenella
quercetin
-
Eimeria tenella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
D-glucose wild-type, pH 9.0, 36°C Eimeria tenella
5.66
-
2-deoxy-D-glucose wild-type, pH 9.0, 36°C Eimeria tenella
13.76
-
D-fructose wild-type, pH 9.0, 36°C Eimeria tenella
16.9
-
D-glucose mutant D217H, pH 9.0, 36°C Eimeria tenella
25.41
-
D-mannose wild-type, pH 9.0, 36°C Eimeria tenella
26.4
-
D-glucose mutant N216K, pH 9.0, 36°C Eimeria tenella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
-
Eimeria tenella
50500
-
-
Eimeria tenella

Organism

Organism UniProt Comment Textmining
Eimeria tenella A0A172WCE4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
oocyte
-
Eimeria tenella
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose
-
Eimeria tenella ADP + 2-deoxy-D-glucose 6-phosphate
-
?
ATP + D-fructose
-
Eimeria tenella ADP + D-fructose 6-phosphate
-
?
ATP + D-glucose
-
Eimeria tenella ADP + D-glucose 6-phosphate
-
?
ATP + D-mannose poor substrate Eimeria tenella ADP + D-mannose 6-phosphate
-
?
additional information no substrate: D-galactose Eimeria tenella ?
-
?

Subunits

Subunits Comment Organism
? x * 50500, calculated, x * 50000, SDS-PAGE Eimeria tenella

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
-
Eimeria tenella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
D-mannose wild-type, pH 9.0, 36°C Eimeria tenella
1.5
-
D-fructose wild-type, pH 9.0, 36°C Eimeria tenella
10.8
-
D-glucose mutant N216K, pH 9.0, 36°C Eimeria tenella
33.7
-
2-deoxy-D-glucose wild-type, pH 9.0, 36°C Eimeria tenella
39.7
-
D-glucose mutant D217H, pH 9.0, 36°C Eimeria tenella
219
-
D-glucose wild-type, pH 9.0, 36°C Eimeria tenella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Eimeria tenella

pI Value

Organism Comment pI Value Maximum pI Value
Eimeria tenella calculated
-
5.7

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.007
-
pH 9.0, 36°C Eimeria tenella quercetin
0.086
-
pH 9.0, 36°C Eimeria tenella mangiferin