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Literature summary for 2.7.1.1 extracted from

  • Khu, L.Y.; Storey, K.B.; Rubtsov, A.M.; Goncharova, N.Y.
    Regulation of glucokinase activity in liver of hibernating ground squirrel Spermophilus undulatus (2014), Biochemistry (Moscow), 79, 727-732.
    View publication on PubMed
Search for more literature for 2.7.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 D-glucose enzyme from active animals, S0.5 value, Hill coefficient 1.16, pH 7.8, 2°C Urocitellus undulatus
6.23
-
 D-glucose enzyme from active animals, S0.5 value, Hill coefficient 1.7, pH 7.8, 25°C Urocitellus undulatus
9
-
 D-glucose enzyme from hibernating animals, S0.5 value, Hill coefficient 1.6, pH 7.8, 25°C Urocitellus undulatus
45
-
 D-glucose enzyme from hibernating animals, S0.5 value, Hill coefficient 1.2, pH 7.8, 2°C Urocitellus undulatus

Organism

Organism UniProt Comment Textmining
Urocitellus undulatus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Urocitellus undulatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Urocitellus undulatus ADP + D-glucose 6-phosphate
-
 ?

General Information

General Information Comment Organism
physiological function entrance of ground squirrels into hibernation from their active state is accompanied by a sharp decrease in blood glucose level (from 14 to 2.9 mM) and with a 7fold decrease of glucokinase activity in the liver cytoplasm. The dependence of glucokinase activity upon glucose concentration for the enzyme from active ground squirrel liver shows a pronounced sigmoid character (Hill coefficient, h = 1.70 and S0.5 = 6.23 mM). On decreasing the temperature to 2°C (simulation of hibernation conditions), this dependency becomes almost hyperbolic (h = 1.16) and glucokinase affinity for substrate is reduced (S0.5 = 23 mM). These parameters for hibernating ground squirrels (body temperature 5°C) at 25°C are practically equal to the corresponding values obtained for glucokinase from the liver of active animals Urocitellus undulatus