Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol 3-phosphate | influences enzyme activity at pH 6.5 by preventing substrate and product inhibition by ATP and ADP, respectively | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Trypanosoma brucei | |
quercetin | quercetin inhibition, but not binding, is relieved by glycerol 3-phosphate at pH 6.5 | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
ATP | pH 6.5, temperature not specified in the publication | Trypanosoma brucei | |
0.28 | - |
ATP | pH 7.4, temperature not specified in the publication | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using affinity and ion-exchange chromatography | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Trypanosoma brucei | ADP + D-glucose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hexokinase | - |
Trypanosoma brucei |
TbHK1 | - |
Trypanosoma brucei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
enzyme shows no activity | Trypanosoma brucei |
7 | - |
50% activity | Trypanosoma brucei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
hexokinase activity is pH-sensitive, but glycerol 3-phosphate prevents inactivation even maintaining detectable enzyme activity at pH 4.0 | Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
4.2 | - |
pH 6.5, temperature not specified in the publication | Trypanosoma brucei | ADP | |
10 | - |
pH 7.4, temperature not specified in the publication | Trypanosoma brucei | ADP |