Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 1 mM, 92% inhibition. Competitive inhibition versus ATP, noncompetitive inhibition versus D-glucose | Sulfurisphaera tokodaii | |
AMP | 1 mM, 73% inhibition | Sulfurisphaera tokodaii | |
CDP | 1 mM, 33% inhibition | Sulfurisphaera tokodaii | |
D-fructose 6-phosphate | 10 mM, 33% inhibition | Sulfurisphaera tokodaii | |
diphosphate | 10 mM, 54% inhibition | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-glucose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
0.12 | - |
ATP | pH 7.5, 50°C, D-glucose as phosphoryl acceptor | Sulfurisphaera tokodaii | |
0.13 | - |
D-mannose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
0.23 | - |
D-glucosamine | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
0.32 | - |
N-acetyl-D-glucosamine | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
0.44 | - |
2-deoxy-D-glucose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii | |
0.52 | - |
CTP | pH 7.5, 50°C, D-glucose as phosphoryl acceptor | Sulfurisphaera tokodaii | |
30 | - |
D-fructose | pH 7.5, 50°C, ATP as phosphoryl donor | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM | Sulfurisphaera tokodaii | |
Mg2+ | no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM | Sulfurisphaera tokodaii | |
Mn2+ | no activity is detected without a divalent cation. 100, 153, and 80% relative activity in the presence of Mg2+, Co2+, and Mn2+, respectively, at 4 mM | Sulfurisphaera tokodaii | |
additional information | no activity in the presence of Zn2+ | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Sulfurisphaera tokodaii |
32100 | - |
2 * 32100, calculated from sequence | Sulfurisphaera tokodaii |
64000 | - |
gel filtration | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Sulfurisphaera tokodaii | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | Sulfurisphaera tokodaii 7 | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q96Y14 | - |
- |
Sulfurisphaera tokodaii 7 | Q96Y14 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
pH 7.5, 50°C, substrate: D-glucose | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | - |
Sulfurisphaera tokodaii | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + 2-deoxy-D-glucose | - |
Sulfurisphaera tokodaii 7 | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + D-fructose | - |
Sulfurisphaera tokodaii | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-glucosamine | - |
Sulfurisphaera tokodaii | ADP + D-glucosamine 6-phosphate | - |
? | |
ATP + D-glucose | - |
Sulfurisphaera tokodaii | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | Sulfurisphaera tokodaii | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Sulfurisphaera tokodaii 7 | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | in Sulfolobus tokodaii this is the sole enzyme responsible for the phosphorylation of glucose in vivo | Sulfurisphaera tokodaii 7 | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-mannose | - |
Sulfurisphaera tokodaii | ADP + D-mannose 6-phosphate | - |
? | |
ATP + N-acetyl-D-glucosamine | - |
Sulfurisphaera tokodaii | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
CTP + D-glucose | 81% of the activity with ATP | Sulfurisphaera tokodaii | CDP + D-glucose 6-phosphate | - |
? | |
GTP + D-glucose | 52% of the activity with ATP | Sulfurisphaera tokodaii | GDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | 64% of the activity with ATP | Sulfurisphaera tokodaii | IDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | 64% of the activity with ATP | Sulfurisphaera tokodaii 7 | IDP + D-glucose 6-phosphate | - |
? | |
additional information | galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | galactose is phosphorylated at rates of 0.8 and 0.08 U/mg at concentrations of 1 and 10 mM, respectively. Ribose, xylose, N-acetylmannosamine, glucose-1-phosphate, and fructose-6-phosphate (10 mM) are not phosphorylated by the enzyme | Sulfurisphaera tokodaii 7 | ? | - |
? | |
UTP + D-glucose | 37% of the activity with ATP | Sulfurisphaera tokodaii | UDP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Sulfurisphaera tokodaii |
dimer | 2 * 32100, calculated from sequence | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent hexokinase | - |
Sulfurisphaera tokodaii |
STK_23540 | locus name | Sulfurisphaera tokodaii |
StoHK | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
pH 7.5, 120 min, 14% loss of activity | Sulfurisphaera tokodaii |
90 | - |
pH 7.5, 120 min, 80% loss of activity | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | more than 60% of the maximal activity in the pH range of 6.5 to 9.0 | Sulfurisphaera tokodaii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
ADP | pH 7.5, 50°C | Sulfurisphaera tokodaii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
D-fructose | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
52 | - |
CTP | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
100 | - |
N-acetyl-D-glucosamine | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
170 | - |
2-deoxy-D-glucose | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
247 | - |
D-glucosamine | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
259 | - |
ATP | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
420 | - |
D-mannose | pH 7.5, 50°C | Sulfurisphaera tokodaii | |
717 | - |
D-glucose | pH 7.5, 50°C | Sulfurisphaera tokodaii |