Literature summary for 2.7.1.1 extracted from

Morris, M.T.; DeBruin, C.; Yang, Z.; Chambers, J.W.; Smith, K.S.; Morris, J.C.
Activity of a second Trypanosoma brucei hexokinase is controlled by an 18-amino-acid C-terminal tail (2006), Eukaryot. Cell, 5, 2014-2023.

Search for more literature for 2.7.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, recombinant TbHK2 lacks detectable enzymatic activity. Replacing the last 18 amino acids of TbHK2 with the corresponding residues of TbHK1 yields an active recombinant protein with kinetic properties similar to those of TbHK1	Trypanosoma brucei
expression in Escherichia coli, TbHK1	Trypanosoma brucei

Protein Variants

Protein Variants	Comment	Organism
additional information	replacing the last 18 amino acids of TbHK1 with the corresponding residues of TbHK2 yields an inactive recombinant protein	Trypanosoma brucei
additional information	replacing the last 18 amino acids of TbHK2 with the corresponding residues of TbHK1 yields an active recombinant protein with kinetic properties similar to those of TbHK1	Trypanosoma brucei
N469D	inactive mutant protein, TbHK1	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
lauric acid	IC50: 0.0758 mM, TbHK1	Trypanosoma brucei
myristic acid	IC50: 0.0784 mM, TbHK1	Trypanosoma brucei
palmitic acid	IC50: 0.0624 mM, TbHK1	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.009	-	D-glucose	recombinant TbHK1	Trypanosoma brucei
0.03	-	D-mannose	recombinant TbHK1	Trypanosoma brucei
0.035	-	D-fructose	recombinant TbHK1	Trypanosoma brucei
0.28	-	ATP	recombinant TbHK1	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	Q95PL2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2-deoxy-D-glucose	-	Trypanosoma brucei	ADP + 2-deoxy-D-glucose 6-phosphate	-	?
ATP + D-glucose	-	Trypanosoma brucei	ADP + D-glucose 6-phosphate	-	?
D-fructose + ATP	-	Trypanosoma brucei	ADP + D-fructose 6-phosphate	-	?
D-glucosamine + ATP	-	Trypanosoma brucei	ADP + D-glucosamine 6-phosphate	-	?
D-mannose + ATP	-	Trypanosoma brucei	ADP + D-mannose 6-phosphate	-	?
additional information	the C-terminal tail of TbHK1 is important for hexokinase activity	Trypanosoma brucei	?	-	?

Synonyms

Synonyms	Comment	Organism
TbHK1	-	Trypanosoma brucei
TbHK2	-	Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
483.3	-	D-glucose	recombinant TbHK1	Trypanosoma brucei

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0624	-	IC50: 0.0624 mM, TbHK1	Trypanosoma brucei	palmitic acid
0.0758	-	IC50: 0.0758 mM, TbHK1	Trypanosoma brucei	lauric acid
0.0784	-	IC50: 0.0784 mM, TbHK1	Trypanosoma brucei	myristic acid

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Release 2023.1 (January 2023)