Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y214C | the glucose binding affinity is increased while its cooperativity is decreased | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | cosubstrate: glucose, wild-type enzyme | Homo sapiens | |
0.23 | - |
D-glucose | mutant enzyme Y214C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinantly expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | - |
Homo sapiens | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
2-deoxy-D-glucose | wild-type enzyme | Homo sapiens | |
38 | - |
D-glucose | wild-type enzyme | Homo sapiens | |
39 | - |
D-glucose | mutant enzyme Y214C | Homo sapiens |