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Literature summary for 2.7.1.1 extracted from
- Multiplex inhibitor screening and kinetic constant determinations for yeast hexokinase using mass spectrometry based assays (2003), J. Am. Soc. Mass Spectrom., 14, 173-181.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive versus ATP, inhibition mode, 91.3% decreased activity | Saccharomyces cerevisiae |  |
| AMP | 51.4% decreased activity | Saccharomyces cerevisiae | |
| CoA | 54.1% decreased activity | Saccharomyces cerevisiae | |
| D-glucose 1,6-diphosphate | 15.7% decreased activity | Saccharomyces cerevisiae | |
| D-glucose 6-phosphate | 54.3% decreased activity | Saccharomyces cerevisiae | |
| GDP | 31.3% decreased activity | Saccharomyces cerevisiae | |
| additional information | multiplex inhibitor screening, mass spectrometry-based assay | Saccharomyces cerevisiae | |
| oxidized glutathione | 82.9% decreased activity | Saccharomyces cerevisiae | |
| reduced glutathione | 54.5% decreased activity | Saccharomyces cerevisiae | |
| trehalose 6-phosphate | 90.9% decreased activity | Saccharomyces cerevisiae | |
| UDP | 51.9% decreased activity | Saccharomyces cerevisiae | |
| UDP-glucose | 62.5% decreased activity | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, mass spectrometry-based assay, overview, the Mg2+ concentration influence the kinetics | Saccharomyces cerevisiae | |
| 0.1 | - |
ATP | pH 7.5, 22°C | Saccharomyces cerevisiae | |
| 0.13 | - |
D-glucose | pH 7.5, 22°C | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | Saccharomyces cerevisiae | - |
ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-glucose | Saccharomyces cerevisiae | first step in the process of glycolysis | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | Saccharomyces cerevisiae | - |
ADP + D-mannose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Saccharomyces cerevisiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
| 120 | - |
commercial preparation | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Saccharomyces cerevisiae | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Saccharomyces cerevisiae | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | first step in the process of glycolysis | Saccharomyces cerevisiae | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | - |
Saccharomyces cerevisiae | ADP + D-mannose 6-phosphate | - |
? | |
| additional information | relatively nonspecific enzyme | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-D-hexose-6-phosphotransferase | - |
Saccharomyces cerevisiae |
| HK | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.06 | - |
D-glucose | pH 7.5, 22°C, soluble enzyme | Saccharomyces cerevisiae | |
| 1.67 | - |
D-glucose | pH 7.5, 22°C, immobilized enzyme | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.122 | - |
ADP | pH 7.5, 22°C, versus ATP, dependennt on Mg2+ concentration | Saccharomyces cerevisiae | |
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