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Literature summary for 2.7.1.1 extracted from
- Isolation and functional characterization of a novel plastidic hexokinase from Nicotiana tabacum (2005), FEBS Lett., 579, 827-831.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme hexokinase 2, promotor isolation and analysis, in vitro transcription and translation, and import of intact precursor proteins, native or fused to GFP, into stroma of chloroplasts of spinach or pea, expression in transgenic tobacco plants via Agrobacterium tumefaciens infection system | Nicotiana tabacum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of transgenic tobacco plants overexpressing isozyme hexokinase 2 via Agrobacterium tumefaciens infection system | Nicotiana tabacum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of isozyme hexokinase 2 | Nicotiana tabacum | |
| 0.025 | - |
D-fructose | pH 8.4, 30°C, recombinant isozyme hexokinase 2 | Nicotiana tabacum |  |
| 0.06 | - |
D-glucose | pH 8.4, 30°C, recombinant isozyme hexokinase 2 | Nicotiana tabacum | |
| 0.149 | - |
D-mannose | pH 8.4, 30°C, recombinant isozyme hexokinase 2 | Nicotiana tabacum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast stroma | hexokinase 2, enzyme contains a putative cleavable chloroplast transit peptide of 30 amino acids | Nicotiana tabacum | 9570 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Nicotiana tabacum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, about, amino acid sequence calculation | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | Nicotiana tabacum | - |
ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-glucose | Nicotiana tabacum | isozyme hexokinase 2 plays a role in starch and secondary metabolism in starch sheath, xylem parenchyma, guard cells, and root tips | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | Nicotiana tabacum | - |
ADP + D-mannose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | Q6Q8A5 | isozyme hexokinase 2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bundle sheath | vascular starch sheath | Nicotiana tabacum | - |
| guard cell | - |
Nicotiana tabacum | - |
| leaf | - |
Nicotiana tabacum | - |
| parenchyma | xylem | Nicotiana tabacum | - |
| root tip | - |
Nicotiana tabacum | - |
| xylem | parenchym | Nicotiana tabacum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Nicotiana tabacum | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Nicotiana tabacum | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | isozyme hexokinase 2 plays a role in starch and secondary metabolism in starch sheath, xylem parenchyma, guard cells, and root tips | Nicotiana tabacum | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-mannose | - |
Nicotiana tabacum | ADP + D-mannose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, about, amino acid sequence calculation | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hexokinase 2 | - |
Nicotiana tabacum |
| Hxk2 | - |
Nicotiana tabacum |
| More | enzyme is a type A hexokinase | Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana tabacum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Nicotiana tabacum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 9.2 | - |
Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Nicotiana tabacum | |
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