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Literature summary for 2.7.1.1 extracted from
- Kinetic studies of rat liver hexokinase D ('glucokinase') in non-co-operative conditions show an ordered mechanism with MgADP as the last product to be released (2003), Biochem. J., 371, 29-38.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-D-glucose | low substrate inhibition | Rattus norvegicus |  |
| 5,5'-dithiobis-(2-nitrobenzoic acid) | inactivation, protection by MgADP-, AMP, 2-deoxyglucose, glucose, and mannose probably via binary complex formation, no protection by glucose 6-phosphate, slight protection by MgATP2- | Rattus norvegicus | |
| AMP | binds to free enzyme and to D-glucose-enzyme | Rattus norvegicus | |
| Cr(III)-ATP | complex of ATP with chromium in the 3+ oxidation state, mixed versus MgATP2-, competitive inhibition versus 2-deoxyglucose | Rattus norvegicus | |
| MgADP- | product inhibition, mixed type inhibition versus MgATP2-, competitive inhibition versus 2-deoxyglucose | Rattus norvegicus | |
| additional information | inhibition mechanism, overview | Rattus norvegicus | |
| N-acetylglucosamine | mixed versus MgATP2-, competitive inhibition versus 2-deoxyglucose | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | non-cooperative conditions shows an ordered, ternary-complex mechanism with MgADP- as the last product to be released, hyperbolic kinetics with both 2-deoxyglucose and MgATP2- | Rattus norvegicus | |
| 0.56 | - |
ATP | pH 8.0, 30°C | Rattus norvegicus | |
| 0.56 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
| 19.2 | - |
2-deoxy-D-glucose | pH 8.0, 30°C | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Rattus norvegicus | - |
ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from liver, to homogeneity | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-hexose = ADP + D-hexose 6-phosphate | non-cooperative conditions shows an ordered kinetic mechanism with MgADP as the last product to be released | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
partially purified liver enzyme | Rattus norvegicus |
| 70 | - |
purified liver enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-glucose | - |
Rattus norvegicus | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | - |
Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-hexose | - |
Rattus norvegicus | ADP + D-hexose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucokinase | - |
Rattus norvegicus |
| hexokinase D | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | formation of MgATP2- before reaction | Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics, overview | Rattus norvegicus | |
| 0.63 | - |
N-acetylglucosamine | pH 8.0, 30°C, versus 2-deoxyglucose | Rattus norvegicus | |
| 0.8 | - |
N-acetylglucosamine | pH 8.0, 30°C, versus MgATP2- | Rattus norvegicus | |
| 4.5 | - |
Cr(III)-ATP | pH 6.9, 30°C, versus MgATP2- | Rattus norvegicus | |
| 40.7 | - |
2-deoxy-D-glucose | pH 8.0, 30°C, versus MgATP2- | Rattus norvegicus | |
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