Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D158A | naturally occurring mutation, activity and kinetics are similar to the wild-type enzyme | Homo sapiens |
D78A/D158A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Homo sapiens |
G227A/D158A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Homo sapiens |
K102A/D158A | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme | Homo sapiens |
K90A/D158A | site-directed mutagenesis, 2fold increased activity compared to the wild-type enzyme | Homo sapiens |
S336L | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Homo sapiens |
S411A | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Homo sapiens |
S411L | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Homo sapiens |
T228A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Homo sapiens |
T228A/D158A | site-directed mutagenesis, very highly reduced activity compared to the wild-type enzyme | Homo sapiens |
T82A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type and mutant enzymes, cooperative/sigmoidal glucose-dependent kinetics | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | as MgATP2-, binding site structure | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Homo sapiens | the pancreatic enzyme is imporatnt as glucose sensor in insulin secretion and blood glucose homeostasis, interaction of ATP and glucose during binding to the enzyme | ADP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
pancreatic beta cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax of wild-type and mutant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | - |
Homo sapiens | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | the pancreatic enzyme is imporatnt as glucose sensor in insulin secretion and blood glucose homeostasis, interaction of ATP and glucose during binding to the enzyme | Homo sapiens | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecuar structure modeling | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | as MgATP2-, binding site structure, ATP binding involves residues Asp78, Thr82, Gly227, Thr228, and S336 | Homo sapiens |