Cloned (Comment) | Organism |
---|---|
hexokinase gene is cloned and expressed in Escherichia coli BL21, amino acid sequence, single copy gene | Toxoplasma gondii |
General Stability | Organism |
---|---|
one cycle of freezing and thawing, no loss of activity | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by D-fructose 6-phosphate, ADP; not inhibited by D-glucose 6-phosphate; not inhibited by trehalose 6-phosphate | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
D-glucose | at 37°C, pH 7 | Toxoplasma gondii | |
1.05 | - |
ATP | at 37°C, pH 7 | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | in both cytosol and membrane fractions, hexokinase weakly associates with the membrane or particulate fraction | Toxoplasma gondii | 5829 | - |
membrane | in both cytosol and membrane fractions, hexokinase weakly associates with the membrane or particulate fraction | Toxoplasma gondii | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, SDS-PAGE, x * 51465, calculated from the amino acid sequence | Toxoplasma gondii |
51465 | - |
x * 51000, SDS-PAGE, x * 51465, calculated from the amino acid sequence | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | Toxoplasma gondii | first step of the glycolytic pathway, physiological function of membrane-bound hexokinase in the tachyzoite: glucose channeling with a glucose/hexose transporter | D-glucose 6-phosphate + ADP | - |
? | |
D-glucose + ATP | Toxoplasma gondii | rate-limiting step in the glycolysis | D-glucose 6-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q969A8 | RH strain | - |
Toxoplasma gondii RH | Q969A8 | RH strain | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | a posttranslational modification leading to membrane binding may occur | Toxoplasma gondii |
Purification (Comment) | Organism |
---|---|
recombinant hexokinase, expressed in Escherichia coli BL21 | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.04 | - |
recombinant hexokinase, expressed in Escherichia coli BL21, at 37°C, pH 7 | Toxoplasma gondii |
Storage Stability | Organism |
---|---|
4°C, 20 mM Tris-HCl, pH 8, 30% glycerol, 100 mM NaCl, 2 mM CaCl2, 0.1 mM EDTA, 1 mM dithiothreitol, 1 week, about 10% loss of activity | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + ATP | 79% of the activity with glucose | Toxoplasma gondii | ADP + D-fructose 6-phosphate | - |
? | |
D-glucose + ATP | specific for ATP | Toxoplasma gondii | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | preferred specificity for glucose | Toxoplasma gondii | ADP + D-glucose 6-phosphate | - |
? | |
D-glucose + ATP | first step of the glycolytic pathway, physiological function of membrane-bound hexokinase in the tachyzoite: glucose channeling with a glucose/hexose transporter | Toxoplasma gondii | D-glucose 6-phosphate + ADP | - |
? | |
D-glucose + ATP | rate-limiting step in the glycolysis | Toxoplasma gondii | D-glucose 6-phosphate + ADP | - |
? | |
D-mannose + ATP | 55% of the activity with glucose | Toxoplasma gondii | ADP + D-mannose 6-phosphate | - |
? | |
additional information | not: ADP | Toxoplasma gondii | ? | - |
? | |
additional information | not: UTP | Toxoplasma gondii | ? | - |
? | |
additional information | not: diphosphate | Toxoplasma gondii | ? | - |
? | |
additional information | not: D-galactose | Toxoplasma gondii | ? | - |
? | |
additional information | not: ITP | Toxoplasma gondii | ? | - |
? | |
additional information | not: GTP | Toxoplasma gondii | ? | - |
? | |
additional information | not: CTP | Toxoplasma gondii | ? | - |
? | |
additional information | not: ADP | Toxoplasma gondii RH | ? | - |
? | |
additional information | not: UTP | Toxoplasma gondii RH | ? | - |
? | |
additional information | not: diphosphate | Toxoplasma gondii RH | ? | - |
? | |
additional information | not: D-galactose | Toxoplasma gondii RH | ? | - |
? | |
additional information | not: ITP | Toxoplasma gondii RH | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, SDS-PAGE, x * 51465, calculated from the amino acid sequence | Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Toxoplasma gondii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.32 | - |
ATP | at 37°C, pH 7 | Toxoplasma gondii | |
3.47 | - |
D-glucose | at 37°C, pH 7 | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Toxoplasma gondii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | more than 80% of the maximal activity between pH 6.5 and 8 | Toxoplasma gondii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Toxoplasma gondii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Toxoplasma gondii | - |
- |
5.82 |