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Literature summary for 2.7.1.1 extracted from
- Purification and properties of buffalo (Bubalus bubalis) erythrocyte hexokinase (1997), Comp. Biochem. Physiol. B, 118, 395-401.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | - |
Bubalus bubalis |  |
| 2-phosphoglycerate | - |
Bubalus bubalis | |
| 3-phosphoglycerate | - |
Bubalus bubalis | |
| ADP | mixed inhibitor versus both MgATP2- and glucose | Bubalus bubalis | |
| ATP | uncomplexed, competitive to MgATP2-, erythrocyte enzyme | Bubalus bubalis | |
| D-fructose 1,6-diphosphate | - |
Bubalus bubalis | |
| D-fructose 6-phosphate | - |
Bubalus bubalis | |
| D-glucose 1,6-diphosphate | - |
Bubalus bubalis | |
| D-glucose 6-phosphate | strong inhibition, potential physiological effector | Bubalus bubalis | |
| Mg2+ | - |
Bubalus bubalis | |
| phosphate | - |
Bubalus bubalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
D-glucose | at 30°C, pH 8.1 | Bubalus bubalis | |
| 0.024 | - |
D-mannose | at 30°C, pH 8.1 | Bubalus bubalis | |
| 0.116 | - |
D-glucosamine | at 30°C, pH 8.1 | Bubalus bubalis | |
| 0.125 | - |
ATP | MgATP2-, at 30°C, pH 8.1 | Bubalus bubalis | |
| 0.134 | - |
2-deoxy-D-glucose | at 30°C, pH 8.1 | Bubalus bubalis | |
| 1.44 | - |
D-fructose | at 30°C, pH 8.1 | Bubalus bubalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
gel filtration | Bubalus bubalis |
| 200000 | - |
tendency of 100 kDa hexokinases to aggregate | Bubalus bubalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bubalus bubalis | - |
hexokinase I | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 52240fold | Bubalus bubalis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-hexose = ADP + D-hexose 6-phosphate | random sequential mechanism | Bubalus bubalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | only one enzyme form | Bubalus bubalis | - |
| erythrocyte | hexokinase type I | Bubalus bubalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.06 | - |
at 30 °C, pH 8.1 | Bubalus bubalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-deoxy-D-glucose | 30% of the activity with glucose | Bubalus bubalis | ADP + 2-deoxy-D-glucose 6-phosphate | - |
? | |
| D-fructose + ATP | 46.3% of the activity with glucose | Bubalus bubalis | ADP + D-fructose 6-phosphate | - |
? | |
| D-glucosamine + ATP | 44.9% of the activity with glucose | Bubalus bubalis | ADP + D-glucosamine 6-phosphate | - |
? | |
| D-glucose + ATP | MgATP2- | Bubalus bubalis | ADP + D-glucose 6-phosphate | - |
? | |
| D-mannose + ATP | 27.2% of the activity with glucose | Bubalus bubalis | ADP + D-mannose 6-phosphate | - |
? | |
| additional information | not: UTP | Bubalus bubalis | ? | - |
? | |
| additional information | not: N-acetyl-D-glucosamine | Bubalus bubalis | ? | - |
? | |
| additional information | not: D-galactose | Bubalus bubalis | ? | - |
? | |
| additional information | not: GTP | Bubalus bubalis | ? | - |
? | |
| additional information | not: CTP | Bubalus bubalis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bubalus bubalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.6 | - |
Bubalus bubalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bubalus bubalis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
D-glucose 6-phosphate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 0.127 | - |
D-glucose 1,6-diphosphate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 0.88 | - |
D-fructose 6-phosphate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 1.5 | - |
ADP | versus MgATP2-, at 30°C, pH 8.1 | Bubalus bubalis | |
| 3.6 | - |
ATP | at 30°C, pH 8.1 | Bubalus bubalis | |
| 3.6 | - |
ADP | versus glucose, at 30°C, pH 8.1 | Bubalus bubalis | |
| 4.6 | - |
D-fructose 1,6-diphosphate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 14 | - |
2,3-diphosphoglycerate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 14 | - |
2-phosphoglycerate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 17.9 | - |
phosphate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 21 | - |
3-phosphoglycerate | at 30°C, pH 8.1 | Bubalus bubalis | |
| 36 | - |
Mg2+ | at 30°C, pH 8.1 | Bubalus bubalis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bubalus bubalis | isoelectric focusing | 6.8 | 6.3 |
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