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Literature summary for 2.7.1.1 extracted from
- Molecular and biochemical characterization of the hexokinase from the starch-utilizing yeast Schwanniomyces occidentalis (1995), Curr. Genet., 27, 330-338.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hexokinase gene is cloned by cross hybridization with a probe from the Saccharomyces cerevisiae HXK2 gene and characterized, DNA is sequenced | Schwanniomyces occidentalis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion mutant is unable to grow on fructose as the sole carbon source, but still grows on glucose, deletion mutants prevent glucose repression of invertase and maltase | Schwanniomyces occidentalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.98 | - |
D-glucose | hexokinase | Schwanniomyces occidentalis |  |
| 9.3 | - |
D-fructose | hexokinase | Schwanniomyces occidentalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53400 | - |
x * 53400, calculated from the DNA sequence | Schwanniomyces occidentalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schwanniomyces occidentalis | - |
starch-utilizing yeast, strain CBS819, i.e. ATCC2322, 2 isoenzymes: hexokinase and glucokinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hexokinase and glucokinase | Schwanniomyces occidentalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.008 | - |
D-fructose as substrate, hxk mutant | Schwanniomyces occidentalis |
| 0.076 | - |
D-glucose as substrate, hxk mutant | Schwanniomyces occidentalis |
| 0.151 | - |
D-glucose as substrate, wild type hexokinase | Schwanniomyces occidentalis |
| 0.197 | - |
D-fructose as substrate, wild type hexokinase | Schwanniomyces occidentalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + ATP | hexokinase, but not glucokinase | Schwanniomyces occidentalis | ADP + D-fructose 6-phosphate | - |
? | |
| D-glucose + ATP | hexokinase and glucokinase | Schwanniomyces occidentalis | ADP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53400, calculated from the DNA sequence | Schwanniomyces occidentalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
hexokinase is extremely unstable at its isoelectric point | Schwanniomyces occidentalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Schwanniomyces occidentalis | |
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