Cloned (Comment) | Organism |
---|---|
cDNA encoding HK I is cloned and expressed in M+R42 cells | Rattus norvegicus |
HK I+, a modified form of HK I with a centrally located polyalanine insert, and D84A mutants of HK I and HK I+ are expressed in M+R42 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D84A | HK I mutant with 2fold increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, mutation diminishes the ability of phosphate to antagonize inhibition, but has no effect on the inhibition by phosphate at higher concentrations | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-Anhydro-D-glucitol 6-phosphate | HK I and HK I+, the D84A mutant of HK I has 2fold increased Ki, HK I: low millimolar concentrations of phosphate antagonize inhibition by competing for an anion binding site in the N-terminal half of HK I, HK I+: insert abolishes the antagonism of phosphate | Rattus norvegicus | |
D-glucose 6-phosphate | inhibition of HK I, antagonism of inhibition by low millimolar concentrations of phosphate results from competition of this ligands for an anion binding site in the N-terminal half of HK I, mechanism | Rattus norvegicus | |
phosphate | at higher concentrations, binds to a lower affinity site in the C-terminal half, HK I and HK I+ | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | 0.053 | D-glucose | HK I, HKI+ and their D84A mutants | Rattus norvegicus | |
0.45 | 0.47 | ATP | HK I and its D84A mutant | Rattus norvegicus | |
1.3 | 1.4 | ATP | HK I+ and its D84A mutant | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
HK I | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
HK I+, a modified form of HK I | - |
Rattus norvegicus | - |
hexokinase type I | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | - |
Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
additional information | ligand binding sites in the N- and C-terminal halves and interactions between these sites | Rattus norvegicus | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | 6 | 1,5-Anhydro-D-glucitol 6-phosphate | HK I+ and its D84A mutant | Rattus norvegicus | |
16 | - |
1,5-Anhydro-D-glucitol 6-phosphate | HK I | Rattus norvegicus | |
32 | - |
1,5-Anhydro-D-glucitol 6-phosphate | D84A mutant of HK I | Rattus norvegicus |