Crystallization (Comment) | Organism |
---|---|
12 mg/ml purified recombinant enzyme, wild-type or selenomethionine-labeled, in 10 mM Tris-HCl, pH 7.5, 0.4 mM potassium phosphate, 1.21 mM NaCl, 8% PEG 8000, 9% PEG 1000, 10% glycerol, and 4 mM 1-deoxy-D-xylulose, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.96-3.2 A resolution | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate | Escherichia coli | - |
pyridoxine 5'-phosphate + phosphate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A794 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by hydroxylapatite and ion exchange chromatography, and gel filtration | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O | reaction energetics and mechanism, active site structure, substrate binding structure, determination of conformation states of the enzyme during reaction from apo to single occupied transitional binding state and to fully occupied state | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate | - |
Escherichia coli | pyridoxine 5'-phosphate + phosphate + H2O | - |
? | |
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate | 1-deoxy-D-xylulose 5-phosphate binding structure | Escherichia coli | pyridoxine 5'-phosphate + phosphate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PdxJ | - |
Escherichia coli |