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Literature summary for 2.6.99.2 extracted from

  • Yeh, J.I.; Du, S.; Pohl, E.; Cane, D.E.
    Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate (2002), Biochemistry, 41, 11649-11657.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
12 mg/ml purified recombinant enzyme, wild-type or selenomethionine-labeled, in 10 mM Tris-HCl, pH 7.5, 0.4 mM potassium phosphate, 1.21 mM NaCl, 8% PEG 8000, 9% PEG 1000, 10% glycerol, and 4 mM 1-deoxy-D-xylulose, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.96-3.2 A resolution Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamics Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate Escherichia coli
-
pyridoxine 5'-phosphate + phosphate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A794
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme by hydroxylapatite and ion exchange chromatography, and gel filtration Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O reaction energetics and mechanism, active site structure, substrate binding structure, determination of conformation states of the enzyme during reaction from apo to single occupied transitional binding state and to fully occupied state Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate
-
Escherichia coli pyridoxine 5'-phosphate + phosphate + H2O
-
?
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate 1-deoxy-D-xylulose 5-phosphate binding structure Escherichia coli pyridoxine 5'-phosphate + phosphate + H2O
-
?

Synonyms

Synonyms Comment Organism
PdxJ
-
Escherichia coli