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Literature summary for 2.6.99.1 extracted from

  • Livneh, Z.; Elad, D.; Sperling, J.
    Enzymatic insertion of purine bases into depurinated DNA in vitro (1979), Proc. Natl. Acad. Sci. USA, 76, 1089-1093.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.99.1

General Stability

General Stability Organism
complete inactivation after 15 min at 60°C Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Caffeine
-
 Escherichia coli
additional information inhibited by high salt concentration e.g. 1 M NaCl Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli may represent a step in a repair mechanism, i.e. base-insertion repair, whereby apurinic sites, which may occur in double-stranded DNA either due to the removal of damaged purines with specific glycosylases or by spontaneous depurination, are directly filled with the correct missing purine base ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + depurinated DNA closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate Escherichia coli deoxyribose triphosphate + purinated DNA
-
 ?
dGTP + depurinated DNA closed circular PM2 phage DNA, linear T7 phage DNA or Escherichia coli DNA can act as substrate Escherichia coli deoxyribose triphosphate + purinated DNA
-
 ?
dTTP + depurinated DNA lower activity than that of dATP and dGTP, incorporation is supressed in the presence of dATP Escherichia coli deoxyribose triphosphate + purinated DNA
-
 ?
additional information specific insertion of adenine into adenine apurinic sites and guanine into guanine apurinic sites Escherichia coli ?
-
 ?
additional information enzyme does not act on depurinated single-stranded DNA Escherichia coli ?
-
 ?
additional information may represent a step in a repair mechanism, i.e. base-insertion repair, whereby apurinic sites, which may occur in double-stranded DNA either due to the removal of damaged purines with specific glycosylases or by spontaneous depurination, are directly filled with the correct missing purine base Escherichia coli ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Escherichia coli