Literature summary for 2.6.1.B19 extracted from

Dow, G.T.; Gilbert, M.; Thoden, J.B.; Holden, H.M.
Structural investigation on WlaRG from Campylobacter jejuni A sugar aminotransferase (2017), Protein Sci., 26, 586-599 .

Search for more literature for 2.6.1.B19

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in apoform and in complex with dTDP-3-amino-3,6-dideoxy-D-galactose, dTDP-3-acetamido-3,6-dideoxy-D-galactose, and dTDP-3-amino-3,6-dideoxy-D-glucose, hanging drop vapor diffusion method, apoenzyme crystals are grown from 100 mM MOPS, pH 7.0, and 15-18% PEG 3350, at room temperature. Internal aldimine are grown from 100 mM MOPS, pH 7.0, 17-19% PEG 3350, 200 mM KCl, and 1 mM PLP, at room temperature. Crystals of dTDP-Qui3N external aldimine complex are grown from 100 mM MOPS, pH 7.0, 12-15% PEG, 10 mM dTDP-Qui3N, and 1 mM PLP, at room temperature. Crystals of the K184A variant in the presence of dTDP-Qui3N are grown from 100 mM HEPES, pH 7.5, 18-21% PEG 5000, 10 mM dTDP-Qui3N, 200 mM KCl, and 1 mM PLP, at room temperature. Crystals of the K184A variant in the presence of dTDP-Fuc3N are grown from 100 mM MOPS, pH 7.0, 13-17% PEG 5000, 10 mM dTDP-Fuc3N, and 1 mM PLP, at room temperature. X-ray diffraction structure determination and analysis at 1.45-1.7 A resolution	Campylobacter jejuni

Crystallization (Comment)

Organism

purified enzyme in apoform and in complex with dTDP-3-amino-3,6-dideoxy-D-galactose, dTDP-3-acetamido-3,6-dideoxy-D-galactose, and dTDP-3-amino-3,6-dideoxy-D-glucose, hanging drop vapor diffusion method, apoenzyme crystals are grown from 100 mM MOPS, pH 7.0, and 15-18% PEG 3350, at room temperature. Internal aldimine are grown from 100 mM MOPS, pH 7.0, 17-19% PEG 3350, 200 mM KCl, and 1 mM PLP, at room temperature. Crystals of dTDP-Qui3N external aldimine complex are grown from 100 mM MOPS, pH 7.0, 12-15% PEG, 10 mM dTDP-Qui3N, and 1 mM PLP, at room temperature. Crystals of the K184A variant in the presence of dTDP-Qui3N are grown from 100 mM HEPES, pH 7.5, 18-21% PEG 5000, 10 mM dTDP-Qui3N, 200 mM KCl, and 1 mM PLP, at room temperature. Crystals of the K184A variant in the presence of dTDP-Fuc3N are grown from 100 mM MOPS, pH 7.0, 13-17% PEG 5000, 10 mM dTDP-Fuc3N, and 1 mM PLP, at room temperature. X-ray diffraction structure determination and analysis at 1.45-1.7 A resolution

Campylobacter jejuni

Protein Variants

Protein Variants	Comment	Organism
K184A	site-directed mutagenesis, structure comparison	Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dTDP-3-dehydro-6-deoxy-D-galactose + D-glutamate	Campylobacter jejuni	-	dTDP-3-amino-3,6-dideoxy-D-galactose + 2-oxoglutarate	-	?
dTDP-3-dehydro-6-deoxy-D-glucose + D-glutamate	Campylobacter jejuni	-	dTDP-3-amino-3,6-dideoxy-D-glucose + 2-oxoglutarate	-	?

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	Q9ALS9	Penner serotype HS	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dTDP-3-dehydro-6-deoxy-D-galactose + D-glutamate	-	Campylobacter jejuni	dTDP-3-amino-3,6-dideoxy-D-galactose + 2-oxoglutarate	-	?
dTDP-3-dehydro-6-deoxy-D-glucose + D-glutamate	-	Campylobacter jejuni	dTDP-3-amino-3,6-dideoxy-D-glucose + 2-oxoglutarate	-	?

Synonyms

Synonyms	Comment	Organism
WlaRG	-	Campylobacter jejuni

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Campylobacter jejuni

General Information

General Information	Comment	Organism
additional information	analysis of external aldimine structures of WlaRG solved in the presence of either dTDP-3-amino-3,6-dideoxy-D-galactose or dTDP-3-amino-3,6-dideoxy-D-glucose	Campylobacter jejuni
physiological function	Campylobacter jejuni 81116 (Penner serotype HS:6) lipoglycan contains two dideoxyhexosamine residues, and this strain can synthesize both dTDP-3-acetamido-3,6-dideoxy-D-glucose and dTDP-3-acetamido-3,6-dideoxy-D-galactose involving enzyme WlaRG, which plays a key role in the production of these unusual sugars by functioning as a pyridoxal 5'-phosphate dependent aminotransferase activity	Campylobacter jejuni