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Literature summary for 2.6.1.B16 extracted from

  • Payer, S.; Schrittwieser, J.; Kroutil, W.
    Vicinal diamines as smart cosubstrates in the transaminase-catalyzed asymmetric amination of ketones (2017), Eur. J. Org. Chem., 2017, 2553-2559 .
No PubMed abstract available
Search for more literature for 2.6.1.B16

Activating Compound

Activating Compound Comment Organism Structure
isooctane
-
 Chromobacterium violaceum
methyl tert-butyl ether
-
 Chromobacterium violaceum
Toluene
-
 Chromobacterium violaceum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli BL21(DE3) Chromobacterium violaceum

General Stability

General Stability Organism
the monomer-dimer equilibrium has an effect on enzyme storage stability. After 3-4 days of incubation, the enzyme activity starts to decrease at the same time as monomers start to show up in the BN-PAGE Chromobacterium violaceum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-1-phenylethylamine + pyruvate Chromobacterium violaceum
-
 acetophenone + L-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and desalting gel filtration Chromobacterium violaceum

Storage Stability

Storage Stability Organism
-20-4°C, purified recombinant enzyme in 50 mM HEPES, pH 8.2, stored for six months in 50% glycerol is still fully active (114%) and the same solution stored at room temperature maintains 77% activity Chromobacterium violaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-1-phenylethylamine + pyruvate
-
 Chromobacterium violaceum acetophenone + L-alanine
-
 r
additional information application of the amine transaminase (ATA) for stereoselective amination of prochiral ketones represents an environmentally benign and economically attractive alternative to transition metal catalyzed asymmetric synthesis, overview Chromobacterium violaceum ?
-
-

Subunits

Subunits Comment Organism
homodimer
-
 Chromobacterium violaceum
More the monomer-dimer equilibrium has an effect on enzyme storage stability. After 3-4 days of incubation, the enzyme activity starts to decrease at the same time as monomers start to show up in the BN-PAGE. Cofactor has a crucial role in dimerstabilization Chromobacterium violaceum

Synonyms

Synonyms Comment Organism
amine transaminase
-
 Chromobacterium violaceum
ATA
-
 Chromobacterium violaceum
Cv-ATA
-
 Chromobacterium violaceum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Chromobacterium violaceum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 effects of co-solvents, i.e. DMSO, glycerol, and methanol, as well as of NaCl and sucrose on the Tm value of the enzyme, overview. DMSO, NaCl, and methanol, and also phenylethylamine and L-alanine reduce the Tm value, while pyruvate, glycerol, and sucrose increase it. The addition of amine substrates (L-alanine and (S)-1-PEA) displaying destabilizing effects may result from PMP (the reduced amine form of the cofactor PLP) formation. PMP is not covalently bound to the enzyme's active site and may therefore promote enzyme destabilization or denaturation Chromobacterium violaceum
78
-
 the enzyme displays a high Tm of 78°C in 100 mM HEPES, pH 7.4, and 100 mM NaCl Chromobacterium violaceum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Chromobacterium violaceum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate PLP has a crucial role in dimerstabilization Chromobacterium violaceum