Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isooctane | - |
Chromobacterium violaceum | |
methyl tert-butyl ether | - |
Chromobacterium violaceum | |
Toluene | - |
Chromobacterium violaceum |
Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli BL21(DE3) | Chromobacterium violaceum |
General Stability | Organism |
---|---|
the monomer-dimer equilibrium has an effect on enzyme storage stability. After 3-4 days of incubation, the enzyme activity starts to decrease at the same time as monomers start to show up in the BN-PAGE | Chromobacterium violaceum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethylamine + pyruvate | Chromobacterium violaceum | - |
acetophenone + L-alanine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Chromobacterium violaceum |
Storage Stability | Organism |
---|---|
-20-4°C, purified recombinant enzyme in 50 mM HEPES, pH 8.2, stored for six months in 50% glycerol is still fully active (114%) and the same solution stored at room temperature maintains 77% activity | Chromobacterium violaceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethylamine + pyruvate | - |
Chromobacterium violaceum | acetophenone + L-alanine | - |
r | |
additional information | application of the amine transaminase (ATA) for stereoselective amination of prochiral ketones represents an environmentally benign and economically attractive alternative to transition metal catalyzed asymmetric synthesis, overview | Chromobacterium violaceum | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Chromobacterium violaceum |
More | the monomer-dimer equilibrium has an effect on enzyme storage stability. After 3-4 days of incubation, the enzyme activity starts to decrease at the same time as monomers start to show up in the BN-PAGE. Cofactor has a crucial role in dimerstabilization | Chromobacterium violaceum |
Synonyms | Comment | Organism |
---|---|---|
amine transaminase | - |
Chromobacterium violaceum |
ATA | - |
Chromobacterium violaceum |
Cv-ATA | - |
Chromobacterium violaceum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Chromobacterium violaceum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
effects of co-solvents, i.e. DMSO, glycerol, and methanol, as well as of NaCl and sucrose on the Tm value of the enzyme, overview. DMSO, NaCl, and methanol, and also phenylethylamine and L-alanine reduce the Tm value, while pyruvate, glycerol, and sucrose increase it. The addition of amine substrates (L-alanine and (S)-1-PEA) displaying destabilizing effects may result from PMP (the reduced amine form of the cofactor PLP) formation. PMP is not covalently bound to the enzyme's active site and may therefore promote enzyme destabilization or denaturation | Chromobacterium violaceum |
78 | - |
the enzyme displays a high Tm of 78°C in 100 mM HEPES, pH 7.4, and 100 mM NaCl | Chromobacterium violaceum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | PLP has a crucial role in dimerstabilization | Chromobacterium violaceum |