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Literature summary for 2.6.1.99 extracted from

  • He, W.; Brumos, J.; Li, H.; Ji, Y.; Ke, M.; Gong, X.; Zeng, Q.; Li, W.; Zhang, X.; An, F.; Wen, X.; Li, P.; Chu, J.; Sun, X.; Yan, C.; Yan, N.; Xie, D.Y.; Raikhel, N.; Yang, Z.; Stepanova, A.N.; Alonso, J.M.; Guo, H.
    A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis (2011), Plant Cell, 23, 3944-3960.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-TAA1 in Escherichia coli strain BL21 Star (DE3) pLysS Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
L-kynurenine an alternate substrate, competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions. L-kynurenine application represses activity and nuclear accumulation of the EIN3 transcription factor in roots. L-kynurenine effectively and selectively binds to the substrate pocket of TAA1/TAR proteins but not to those of other families of aminotransferases, computational docking and molecular modeling, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + pyruvate Arabidopsis thaliana
-
 indole-3-pyruvate + L-alanine
-
 ?
L-tryptophan + pyruvate Arabidopsis thaliana Col-0
-
 indole-3-pyruvate + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9S7N2
-
-
Arabidopsis thaliana Col-0 Q9S7N2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + pyruvate
-
 Arabidopsis thaliana indole-3-pyruvate + L-alanine
-
 ?
L-tryptophan + pyruvate
-
 Arabidopsis thaliana Col-0 indole-3-pyruvate + L-alanine
-
 ?
additional information L-kynurenine is an alternate substrate competing with L-tryptophan Arabidopsis thaliana ?
-
 ?
additional information L-kynurenine is an alternate substrate competing with L-tryptophan Arabidopsis thaliana Col-0 ?
-
 ?

Synonyms

Synonyms Comment Organism
TAA1
-
 Arabidopsis thaliana
tryptophan aminotransferase of Arabidopsis 1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01152
-
 L-kynurenine inhibition of TAA1, competitive versus L-Tryp, pH not specificied in the publication, 55°C Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction L-kynurenine, being an alternate substrate, competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions Arabidopsis thaliana
metabolism tryptophan aminotransferase of Arabidopsis 1 and tryptopan aminotransferase related, i.e. TAA1 and TAR, are the key enzymes in the indole-3-pyruvic acid pathway of auxin biosynthesis Arabidopsis thaliana