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Literature summary for 2.6.1.95 extracted from
- Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin (2007), ChemBioChem, 8, 283-288.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces fradiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48060 | - |
Neo-18, calculated from amino acid sequence | Streptomyces fradiae |
| 48060 | - |
Neo-18, electrospray ionization mass spectrometry | Streptomyces fradiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces fradiae | - |
- |
- |
| Streptomyces fradiae NCIMB 8233 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA resin column chromatography | Streptomyces fradiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| neomycin + 2-oxoglutarate | - |
Streptomyces fradiae | 6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate | - |
? |  |
| neomycin + 2-oxoglutarate | - |
Streptomyces fradiae NCIMB 8233 | 6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6'-oxoglucosaminyl:L-glutamate aminotransferase | - |
Streptomyces fradiae |
| Neo-18 | - |
Streptomyces fradiae |
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Release 2023.1 (January 2023)
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