Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3) | Burkholderia lata |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method, PEG 3350 | Burkholderia lata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | reduced activity | Burkholderia lata | |
Ni2+ | reduced activity | Burkholderia lata | |
Zn2+ | reduced activity | Burkholderia lata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
chorismate | pH 7.0, 25°C | Burkholderia lata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Burkholderia lata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals | Burkholderia lata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia lata | - |
- |
- |
Burkholderia lata 383 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), cleavage of the tag, gel filtration | Burkholderia lata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + chorismate | - |
Burkholderia lata | (S)-2-amino-2-deoxyisochorismate + L-glutamate | - |
? | |
L-glutamine + chorismate | - |
Burkholderia lata 383 | (S)-2-amino-2-deoxyisochorismate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 70000, gel filtration, 140000 Da homodimer found in the asymmetric unit of crystals | Burkholderia lata |
Synonyms | Comment | Organism |
---|---|---|
2-amino-2desoxyisochorismate synthase | - |
Burkholderia lata |
PhzE | - |
Burkholderia lata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
chorismate | pH 7.0, 25°C | Burkholderia lata |