Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-hydroxyphenazine | inhibitory above 0.200 mM | Pseudomonas aeruginosa | |
additional information | enzyme is not regulated by feedback inhibition. Little or no inhibition up to 1 mM: glyphosate, pyridoxamine 5'-phosphate, pyridoxal 5'-phosphate, and 3-hydroxypyridine-4-carboxylic acid | Pseudomonas aeruginosa | |
phosphate | phosphate buffer competitively inhibits PhzE, about 20% inhibition with 100 mM potassium phosphate at pH 7.0 | Pseudomonas aeruginosa | |
pyocyanin | inhibitory above 0.250 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
chorismate | cosubstrate L-glutamine, pH 7.0, 25°C | Pseudomonas aeruginosa | |
0.216 | - |
chorismate | cosubstrate L-glutamine, pH 7.0, 25°C | Pseudomonas aeruginosa | |
11 | - |
L-glutamine | pH 7.0, 25°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Pseudomonas aeruginosa | |
Mg2+ | required, Km value 0.29 mM | Pseudomonas aeruginosa | |
Mn2+ | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71251 | - |
- |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q7DC81 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-glutamine | - |
Pseudomonas aeruginosa | L-glutamate + 4-amino-4-deoxychorismate | - |
? | |
chorismate + NH3 | - |
Pseudomonas aeruginosa | 4-amino-4-deoxychorismate + H2O | - |
? | |
additional information | isoform PhzE catalyzes the addition of ammonia to C2 of chorismate, as does anthranilate synthase, yet unlike anthranilate synthase it does not catalyze elimination of pyruvate from enzyme-bound aminodeoxyisochorismate. Reaction follows a random kinetic mechanism, enzyme PhzE can use NH4+ as an alternative nucleophile. PhzE shows no lyase activity | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71274, calculated, x * 71251, MALDI-TOF, His-tagged recombinant protein | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
PhzE | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20% of maximum activtiy | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
chorismate | cosubstrate NH3, pH 7.0, 25°C | Pseudomonas aeruginosa | |
2.2 | - |
chorismate | cosubstrate L-glutamine, pH 7.0, 25°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas aeruginosa |