Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chorismate | about 50% activation at 0.002 mM | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
His-tagged protein (first 43 amino acid residues deleted) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NH3 | inhibitor of the glutamine amidotransferase reaction (Ki = 5 mM, lowered to 0.27 mM if considered that NH3 and not NH4+ is the true inhibitor (pKa value of 9.24)) | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
chorismate | apparent K0.5 of chorismate for the activation of glutamine amidotransferase (calculated from chorismate-dependent activation), pH 8.0, 37°C | Arabidopsis thaliana | |
0.0022 | - |
chorismate | p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
0.009 | - |
chorismate | NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
0.045 | - |
L-glutamine | glutamate production in the presence of chorismate, pH 8.0, 37°C | Arabidopsis thaliana | |
0.05 | - |
L-glutamine | glutamate production in the absence of chorismate, pH 8.0, 37°C | Arabidopsis thaliana | |
0.064 | - |
L-glutamine | p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
195 | - |
NH3 | NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Arabidopsis thaliana | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+), gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-glutamine | - |
Arabidopsis thaliana | L-glutamate + 4-amino-4-deoxychorismate | - |
? | |
chorismate + NH3 | - |
Arabidopsis thaliana | 4-amino-4-deoxychorismate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminodeoxychorismate synthase | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
at least stable for 30 min | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
NH3 | NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
0.22 | - |
L-glutamine | p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
0.23 | - |
L-glutamine | glutamate production in the absence of chorismate, pH 8.0, 37°C | Arabidopsis thaliana | |
0.79 | - |
L-glutamine | glutamate production in the presence of chorismate, pH 8.0, 37°C | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
NH3 | inhibitor of the glutamine amidotransferase reaction (Ki lowered to 0.27 mM if considered that NH3 and not NH4+ is the true inhibitor (pKa value of 9.24)), pH 8.0, 37°C | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00061 | - |
NH3 | NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
3.4 | - |
L-glutamine | p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
4.6 | - |
L-glutamine | glutamate production in the absence of chorismate, pH 8.0, 37°C | Arabidopsis thaliana | |
13.3 | - |
chorismate | NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana | |
17.5 | - |
L-glutamine | glutamate production in the presence of chorismate, pH 8.0, 37°C | Arabidopsis thaliana | |
100 | - |
chorismate | p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C | Arabidopsis thaliana |