Literature summary for 2.6.1.85 extracted from

Bulloch, E.M.M.; Jones, M.A.; Parker, E.J.; Osborne, A.P.; Stephens, E.; Davies, G.M.; Coggins, J.R.; Abell, C.
Identification of 4-amino-4-deoxychorismate synthase as the molecular target for the antimicrobial action of (6S)-6-fluoroshikimate (2004), J. Am. Chem. Soc., 126, 9912-9913.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2-fluorochorismate	irreversible, covalent modification of K274	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate	omega-amino group of K274 adds to chorismate at C2, leading to elimination of the C4 hydroxyl in an SN2'' displacement reaction. After attack of ammonia to intermediate, another SN2'' displacement expels K274 and forms aminodeoxychorismate	Escherichia coli	a

Subunits

Subunits	Comment	Organism
dimer	heterodimer of chorismate aminating subunit and glutamine amidotransferase subunit. Mass of glutamine amidotransferase subunit is 50969 Da, as determined by electrospray mass spectrometry	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.13	-	2-fluorochorismate	-	Escherichia coli

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Release 2023.1 (January 2023)