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Literature summary for 2.6.1.85 extracted from

  • He, Z.; Stigers Lavoie, K.D.; Bartlett, P.A.; Toney, M.D.
    Conservation of mechanism in three chorismate-utilizing enzymes (2004), J. Am. Chem. Soc., 126, 2378-2385.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression with His-tag Escherichia coli

Protein Variants

Protein Variants Comment Organism
K274A 265fold reduction of kcat-value Escherichia coli
K274R 640fold reduction of kcat-value Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate transition-state analogue, competitive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
chorismate wild-type, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli
0.058
-
chorismate wild-type, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
0.097
-
chorismate mutant K274A, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
145
-
NH4+ wild-type, pH 8.5, 25°C Escherichia coli
169
-
NH4+ mutant K274A, pH 8.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate mechanism includes nucleophile addition to C2 of chorismate in an SN2'' process Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-glutamine
-
Escherichia coli 4-amino-4-deoxychorismate + L-glutamate
-
?
chorismate + NH4+
-
Escherichia coli 4-amino-4-deoxychorismate + H2O + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000098
-
chorismate mutant K274R, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
0.00083
-
chorismate mutant K274R, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli
0.002
-
chorismate mutant K274A, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli
0.026
-
chorismate mutant K274A, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
0.032
-
chorismate wild-type, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
0.53
-
chorismate wild-type, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0007
-
(5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate mutant K274A, pH 8.5, 25°C Escherichia coli
0.0056
-
(5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate wild-type, pH 8.5, 25°C Escherichia coli