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Literature summary for 2.6.1.85 extracted from

  • Viswanathan, V.K.; Green, J.M.; Nicholas, B.P.
    Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli (1995), J. Bacteriol., 177, 5918-5923.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
glutamine prevents loss of activity Escherichia coli
pABA
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by ATP, AMP, dAMP, guanosine, GMP, dGMP, GTP, deoxyguanosine, dTMP, serine, glycine, tyrosine, methionine, p-hydroxybenzoate, PABA, sulfanilamide Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chorismate + L-glutamine Escherichia coli
-
 4-amino-4-deoxychorismate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin sulfate precipitation, ammonium sulfate fractionation, chromatography on DEAE-cellulose Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate the enzyme is composed of two parts, PabA and PabB Escherichia coli
a
chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate in the absence of PabA and glutamine, PabB converts ammonia and chorismate into 4-amino-4-deoxychorismate (in the presence of Mg2+) Escherichia coli
a
chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate non linear production of PABA in the ADC synthase-ADC lyase-coupled reaction system, at different glutamine and chorismate concentrations, with limiting PabB Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-glutamine
-
 Escherichia coli 4-amino-4-deoxychorismate + L-glutamate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 inactivation. DTT, chorismate or PabA prevent inactivation Escherichia coli