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Literature summary for 2.6.1.83 extracted from

  • Cavalcanti, J.H.F.; Kirma, M.; Barros, J.A.S.; Quinhones, C.G.S.; Pereira-Lima, I.A.; Obata, T.; Nunes-Nesi, A.; Galili, G.; Fernie, A.R.; Avin-Wittenberg, T.; Araujo, W.L.
    An L,L-diaminopimelate aminotransferase mutation leads to metabolic shifts and growth inhibition in Arabidopsis (2018), J. Exp. Bot., 69, 5489-5506 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.83

Cloned(Commentary)

Cloned (Comment) Organism
gene DAP, genotyping Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information the dapat (aberrant growth death 2 (agd-2)) mutation of Arabidopsis thaliana leads to metabolic shifts and growth inhibition. Transcriptional and two-dimensional (isoelectric focusing/SDS-PAGE) proteome analyses reveal alterations in the abundance of several transcripts and proteins associated with photosynthesis and photorespiration coupled with a high glycine/serine ratio and increased levels of stress-responsive amino acids. Transcriptome changes induced by the DAPAT mutation, quantitative RT-PCR expression analysis, detailed overview. The leaf proteome is affected by the DAPAT mutation Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
LL-2,6-diaminoheptanedioate + 2-oxoglutarate Arabidopsis thaliana
-
 (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 r
LL-2,6-diaminoheptanedioate + 2-oxoglutarate Arabidopsis thaliana Col-0
-
 (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93ZN9
-
-
Arabidopsis thaliana Col-0 Q93ZN9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
LL-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 Arabidopsis thaliana (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 r
LL-2,6-diaminoheptanedioate + 2-oxoglutarate
-
 Arabidopsis thaliana Col-0 (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O
-
 r

Synonyms

Synonyms Comment Organism
DAP
-
 Arabidopsis thaliana
LL-DAPAT
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction Arabidopsis thaliana DAP mutant dapat shows reduced activity of the Lys biosynthesis enzyme L,L-diaminopimelate aminotransferase, physiological and metabolic impacts of impaired Lys biosynthesis, the mutation leads to metabolic shifts and growth inhibition, phenotype, overview. No differences in dark respiration between genotypes are observed, but a lower storage and consumption of starch and sugars is observed in dapat plants, also higher protein turnover but no differences in total amino acids during a diurnal cycle in dapat plants. Biochemical alterations rather than stomatal limitations are responsible for the decreased photosynthesis and growth of the dapat mutant, which mimics stress conditions associated with impairments in the Lys biosynthesis pathway Arabidopsis thaliana
metabolism the enzyme is involved in the lysine pathway, overview. Lysine is synthetized in the chloroplast using aspartate as a precursor. Dihydrodipicolinate synthase (DHDPS) is the first enzyme of lysine biosynthesis and it requires pyruvate export from the cytosol to the chloroplast. Under stress conditions, lysine is exported from the chloroplast to mitochondria to be degraded, and electrons are used as a donor for ATP synthesis Arabidopsis thaliana