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Literature summary for 2.6.1.83 extracted from

  • Hudson, A.O.; Giron, I.; Dobson, R.C.
    Crystallization and preliminary X-ray diffraction analysis of L,L-diaminopimelate aminotransferase (DapL) from Chlamydomonas reinhardtii (2011), Acta Crystallogr. Sect. F, 67, 140-143.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.83

Cloned(Commentary)

Cloned (Comment) Organism
S- and His-tagged protein expressed in Escherichia coli BL21-CodonPlus-RIPL Chlamydomonas reinhardtii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion method, PEG 3350 Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii CC-1690
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography, gel filtration Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
 substrate tetrahydrodipicolinate, pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii
12
-
 substrate L,L-diaminopimelate, pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + tetrahydrodipicolinate + H2O
-
 Chlamydomonas reinhardtii LL-diaminopimelate + 2-oxoglutarate
-
 r
L-glutamate + tetrahydrodipicolinate + H2O
-
 Chlamydomonas reinhardtii CC-1690 LL-diaminopimelate + 2-oxoglutarate
-
 r
LL-diaminopimelate + 2-oxoglutarate
-
 Chlamydomonas reinhardtii L-glutamate + tetrahydrodipicolinate + H2O
-
 r
LL-diaminopimelate + 2-oxoglutarate
-
 Chlamydomonas reinhardtii CC-1690 L-glutamate + tetrahydrodipicolinate + H2O
-
 r

Synonyms

Synonyms Comment Organism
L,L-diaminopimelate aminotransferase
-
 Chlamydomonas reinhardtii