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Literature summary for 2.6.1.77 extracted from
- Biochemical and molecular characterization of taurine:pyruvate transaminase from the anaerobe Bilophila wadsworthia (2000), Eur. J. Biochem., 267, 6841-6848.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis | Bilophila wadsworthia |
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes | Bilophila wadsworthia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.82 | - |
pyruvate | pH 9.0, 35°C | Bilophila wadsworthia |  |
| 7.1 | - |
taurine | pH 9.0, 35°C | Bilophila wadsworthia | |
| 8.1 | - |
Hypotaurine | pH 9.0, 35°C | Bilophila wadsworthia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
4 * 51000, SDS-PAGE | Bilophila wadsworthia |
| 197000 | - |
gel filtration | Bilophila wadsworthia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| taurine + pyruvate | Bilophila wadsworthia | initial step in anaerobic taurine degradation | L-alanine + 2-sulfoacetaldehyde | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bilophila wadsworthia | Q9APM5 | RZATAU | - |
| Bilophila wadsworthia RZATAU | Q9APM5 | RZATAU | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| buffer MOPS, pH 6.5, apparent homogeneity | Bilophila wadsworthia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity depends strongly on growth substrate | Bilophila wadsworthia |
| 1.02 | - |
taurine/formate-grown cells | Bilophila wadsworthia |
| 2.22 | - |
pyruvate-grown cells | Bilophila wadsworthia |
| 35.3 | - |
purified enzyme | Bilophila wadsworthia |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or 22°C, sulfonate buffers MES or MOPS, pH range 6.0-7.0, 22 h, stable | Bilophila wadsworthia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-aminopropanesulfonate + pyruvate | low activity | Bilophila wadsworthia | ? | - |
? | |
| 3-aminopropanesulfonate + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
| beta-alanine + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
| cysteine + pyruvate | low activity | Bilophila wadsworthia | ? | - |
? | |
| cysteine + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
| hypotaurine + pyruvate | i.e. 2-aminoethanesulfinate | Bilophila wadsworthia | ? | - |
? | |
| hypotaurine + pyruvate | purified enzyme | Bilophila wadsworthia | ? | - |
? | |
| hypotaurine + pyruvate | i.e. 2-aminoethanesulfinate | Bilophila wadsworthia RZATAU | ? | - |
? | |
| additional information | no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor | Bilophila wadsworthia | ? | - |
? | |
| additional information | cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates | Bilophila wadsworthia | ? | - |
? | |
| additional information | no activity with 2-oxoglutarate and phenylpyruvate as amino group acceptor | Bilophila wadsworthia RZATAU | ? | - |
? | |
| additional information | cysteate, glutamate and 2-aminoethanephosphonate are poor amino group donor substrates | Bilophila wadsworthia RZATAU | ? | - |
? | |
| taurine + 2-oxobutyrate | - |
Bilophila wadsworthia | 2-sulfoacetaldehyde + 2-aminobutyrate | - |
? | |
| taurine + 2-oxobutyrate | - |
Bilophila wadsworthia RZATAU | 2-sulfoacetaldehyde + 2-aminobutyrate | - |
? | |
| taurine + oxaloacetate | - |
Bilophila wadsworthia | ? | - |
? | |
| taurine + pyruvate | i.e. 2-aminoethanesulfonate | Bilophila wadsworthia | 2-sulfoacetaldehyde + L-alanine | - |
r | |
| taurine + pyruvate | i.e. 2-aminoethanesulfonate | Bilophila wadsworthia RZATAU | 2-sulfoacetaldehyde + L-alanine | - |
r | |
| taurine + pyruvate | initial step in anaerobic taurine degradation | Bilophila wadsworthia | L-alanine + 2-sulfoacetaldehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| additional information | formation of large aggregates depends on storage conditions, freezing at -20°C, or rapid freezing in liquid nitrogen with storage at -20°C increases the 350 kDa form, addition of glycerol decreases the aggregation, a dimer of 95 kDa is preferably formed during storage at 4°C | Bilophila wadsworthia |
| tetramer | 4 * 51000, SDS-PAGE | Bilophila wadsworthia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tPA | - |
Bilophila wadsworthia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Bilophila wadsworthia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | broad optimum | Bilophila wadsworthia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bilophila wadsworthia |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme is unstable in buffers with neutral or alkaline pH value, except for HEPES at pH 7.0 | Bilophila wadsworthia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | activating | Bilophila wadsworthia | |
| pyridoxal 5'-phosphate | pyridoxyl 5'-phosphate is not essentially required | Bilophila wadsworthia | |
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