Literature summary for 2.6.1.76 extracted from

Ikai, H.; Yamamoto, S.
Identification and analysis of a gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase involved in the 1,3-diaminopropane production pathway in Acinetobacter baumannii (1997), J. Bacteriol., 179, 5118-5125.

Application

Application	Comment	Organism
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter calcoaceticus
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter johnsonii
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter lwoffii
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter baumannii
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter genospecies
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter haemolyticus
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter junii
medicine	drug development, Acinetobacter sp. can be part of the normal flora of human skin but also of opportunistic infections like septicemia, pneumonia, endocarditis, meningitis, skin and wound sepsis or urinary tract infections, clinically important isolates show widespread and increasing resistance to many commonly used antibiotics	Acinetobacter radioresistens

Cloned(Commentary)

Cloned (Comment)	Organism
homologs of dat detected by PCR amplification	Acinetobacter calcoaceticus
homologs of dat detected by PCR amplification	Acinetobacter johnsonii
homologs of dat detected by PCR amplification	Acinetobacter lwoffii
homologs of dat detected by PCR amplification	Acinetobacter genospecies
homologs of dat detected by PCR amplification	Acinetobacter haemolyticus
homologs of dat detected by PCR amplification	Acinetobacter junii
homologs of dat detected by PCR amplification	Acinetobacter radioresistens
identification and analysis of the dat gene encoding L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase with Escherichia coli transformants	Acinetobacter baumannii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.46	-	2-oxoglutaric acid	-	Acinetobacter baumannii
4.3	-	L-2,4-diaminobutyric acid	-	Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	4 * 45000, SDS-PAGE	Acinetobacter baumannii
47420	-	deduced from nucleotide sequence	Acinetobacter baumannii
188000	-	native enzyme, gel filtration	Acinetobacter baumannii

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baumannii	-	-	-
Acinetobacter calcoaceticus	-	-	-
Acinetobacter genospecies	-	3 ATCC 19004	-
Acinetobacter haemolyticus	-	ATCC 17906	-
Acinetobacter johnsonii	-	ATCC 17909	-
Acinetobacter junii	-	ATCC 17908	-
Acinetobacter lwoffii	-	ATCC 15309	-
Acinetobacter radioresistens	-	IAM13186	-
Acinetobacter radioresistens IAM13186	-	IAM13186	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, overexpressed in Escherichia coli	Acinetobacter baumannii

Reaction

Reaction	Comment	Organism	Reaction ID
L-2,4-diaminobutanoate + 2-oxoglutarate = L-aspartate 4-semialdehyde + L-glutamate	Involved in the formation of 1,3-diaminopropane in Haemophilus influenzae and Acinetobacter baumannii. A product of the ddc gene that also encodes L-2,4-diaminobutyrate decarboxylase in Acinetobacter baumannii. Differs from EC 2.6.1.46 diaminobutyrate-pyruvate transaminase, which has pyruvate as the amino-group acceptor	Acinetobacter baumannii	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
beta-alanine + 2-oxoglutaric acid	-	Acinetobacter baumannii	?	-	?
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter calcoaceticus	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter johnsonii	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter lwoffii	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter baumannii	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter genospecies	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter haemolyticus	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter junii	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter radioresistens	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	Acinetobacter radioresistens IAM13186	L-glutamic acid + L-aspartic beta-semialdehyde	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter calcoaceticus	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter johnsonii	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter lwoffii	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter baumannii	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter genospecies	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter haemolyticus	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter junii	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter radioresistens	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-glutamic acid + L-aspartic beta-semialdehyde	-	Acinetobacter radioresistens IAM13186	L-2,4-diaminobutyric acid + 2-oxoglutaric acid	-	r
L-lysine + oxaloacetic acid	-	Acinetobacter baumannii	?	-	?
L-ornithine + pyruvic acid	-	Acinetobacter baumannii	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 45000, SDS-PAGE	Acinetobacter baumannii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.25	8.75	-	Acinetobacter baumannii