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Literature summary for 2.6.1.71 extracted from

  • Schmidt, H.; Bode, R.; Birnbaum, D.
    Lysine degradation in Pichia guilliermondii: characterisation of a novel enzyme, L-lysine:pyruvate aminotransferase (1987), J. Basic Microbiol., 27, 595-601.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 80% remaining activity at 1 mM, 7% remaining activity at 10 mM Meyerozyma guilliermondii
CoCl2 9% activity retained at 1 mM, complete inhibition at 10 mM Meyerozyma guilliermondii
CuCl2 complete inhibition at 1 mM or higher Meyerozyma guilliermondii
EDTA 89% activity retained at 1 mM, 36% activity retained at 10 mM Meyerozyma guilliermondii
FeCl2 23% activity retained at 1 mM, 13% activity retained at 10 mM Meyerozyma guilliermondii
HgCl2 40% activity retained at 1 mM, 31% activity retained at 10 mM Meyerozyma guilliermondii
hydroxylamine 37% activity retained at 1 mM, 6% activity retained at 10 mM Meyerozyma guilliermondii
MgCl2 98% activity retained at 1 mM, 22% activity retained at 10 mM Meyerozyma guilliermondii
MnCl2 57% activity retained at 1 mM, 3% activity retained at 10 mM Meyerozyma guilliermondii
additional information KF, KCN, sodium azide, Na2HAsSO4, NaHSO4, 2-mercaptoethanol, dithioerythritol, reduced glutathione, proteinogenic amino acids, none of them has inhibitory effects even at 10 mM Meyerozyma guilliermondii
p-chloromercuribenzoate 5% activity retained at 1 mM, 1% activity retained at 10 mM Meyerozyma guilliermondii
SnCl2 97% activity retained at 1 mM, 40% retained activity at 10 mM Meyerozyma guilliermondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel filtration Meyerozyma guilliermondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + pyruvate Meyerozyma guilliermondii involved in lysine degradation 2-aminoadipate 6-semialdehyde + L-alanine
-
?
L-lysine + pyruvate Meyerozyma guilliermondii H17 involved in lysine degradation 2-aminoadipate 6-semialdehyde + L-alanine
-
?
additional information Meyerozyma guilliermondii the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source ?
-
?
additional information Meyerozyma guilliermondii H17 the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source ?
-
?

Organism

Organism UniProt Comment Textmining
Meyerozyma guilliermondii
-
-
-
Meyerozyma guilliermondii H17
-
-
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation and DEAE-cellulose, partial purification Meyerozyma guilliermondii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
Meyerozyma guilliermondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-amino-2-oxobutyrate + pyruvate at 2% activity compared to L-lysine Meyerozyma guilliermondii 2,4-dioxobutanoate + L-alanine
-
?
4-amino-2-oxobutyrate + pyruvate at 2% activity compared to L-lysine Meyerozyma guilliermondii H17 2,4-dioxobutanoate + L-alanine
-
?
L-lysine + pyruvate
-
Meyerozyma guilliermondii 2-aminoadipate 6-semialdehyde + L-alanine
-
?
L-lysine + pyruvate involved in lysine degradation Meyerozyma guilliermondii 2-aminoadipate 6-semialdehyde + L-alanine
-
?
L-lysine + pyruvate
-
Meyerozyma guilliermondii H17 2-aminoadipate 6-semialdehyde + L-alanine
-
?
L-lysine + pyruvate involved in lysine degradation Meyerozyma guilliermondii H17 2-aminoadipate 6-semialdehyde + L-alanine
-
?
additional information D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates Meyerozyma guilliermondii ?
-
?
additional information the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source Meyerozyma guilliermondii ?
-
?
additional information D-lysine, L-ornithine, DL-gamma-aminobutyrate, L-norleucine, L-norvaline, putrescine, oxaloacetate, 2-oxoglutarate, 2-oxobutyrate, phenylpyruvate, 4-hydroxyphenylpyruvate are not substrates Meyerozyma guilliermondii H17 ?
-
?
additional information the enzyme is strongly derepressed in cells grown on L-lysine as sole nitrogen source Meyerozyma guilliermondii H17 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Meyerozyma guilliermondii
32
-
-
Meyerozyma guilliermondii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 45 50% of maximal activity at 15°C and 45°C Meyerozyma guilliermondii
15 30 activity increases linearly with temperature Meyerozyma guilliermondii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km: 0.04 mM Meyerozyma guilliermondii