Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.7 extracted from

  • Fang, J.; Han, Q.; Li, J.
    Isolation, characterization, and functional expression of kynurenine aminotransferase cDNA from the yellow fever mosquito, Aedes aegypti(1) (2002), Insect Biochem. Mol. Biol., 32, 943-950.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of full length enzyme and truncated version lacking the mitochondrial leader sequence in Sf9 insect cells Aedes aegypti

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion deduced amino acid sequence contains a mitochondrial leader sequence Aedes aegypti 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, truncated recombinant enzyme, SDS-PAGE Aedes aegypti
53000
-
 x * 53000, SDS-PAGE Aedes aegypti
53490
-
 x * 53490, deduced from nucleotide sequence Aedes aegypti
97000
-
 truncated recombinant enzyme, gel filtration Aedes aegypti

Organism

Organism UniProt Comment Textmining
Aedes aegypti Q95VY4 yellow fever mosquito
-

Purification (Commentary)

Purification (Comment) Organism
truncated recombinant enzyme, DEAE-Sepharose, native PAGE Aedes aegypti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-kynurenine + 2-oxoadipate 25% of activity with pyruvate Aedes aegypti kynurenic acid + 2-aminoadipate + H2O
-
 ?
L-kynurenine + 2-oxoglutarate 31% of activity with pyruvate Aedes aegypti kynurenic acid + L-glutamate + H2O
-
 ?
L-kynurenine + oxaloacetate 90% of activity with pyruvate Aedes aegypti kynurenic acid + L-aspartate + H2O
-
 ?
L-kynurenine + pyruvate best amino acceptor Aedes aegypti kynurenic acid + L-alanine + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Aedes aegypti
? x * 53490, deduced from nucleotide sequence Aedes aegypti
dimer 2 * 48000, truncated recombinant enzyme, SDS-PAGE Aedes aegypti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 recombinant enzyme Aedes aegypti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 optima at pH 8.5 and 10.0 Aedes aegypti
10
-
 optima at pH 8.5 and 10.0 Aedes aegypti