Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | in presence of L-norleucine: ping-pong bi-bi inhibition with alternative substrates | Candida guilliermondii var. membranaefaciens | |
L-norvaline | non-competitive inhibitor | Candida guilliermondii var. membranaefaciens | |
L-valine | competitive inhibitor | Candida guilliermondii var. membranaefaciens | |
additional information | 6-aminohexanoic acid, L-isoleucine, L-ornithine, L-lysine are not inhibitors | Candida guilliermondii var. membranaefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
L-norleucine | pH 7.8, 35°C, in the presence of 2-oxoglutarate | Candida guilliermondii var. membranaefaciens | |
2 | - |
2-oxoglutarate | pH 7.8, 35°C, in the presence of L-norleucine or L-leucine | Candida guilliermondii var. membranaefaciens | |
6.6 | - |
L-leucine | in the presence of 2-oxoglutarate | Candida guilliermondii var. membranaefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 * 51000, SDS-PAGE | Candida guilliermondii var. membranaefaciens |
100000 | - |
gel filtration | Candida guilliermondii var. membranaefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + 2-oxoglutarate | Candida guilliermondii var. membranaefaciens | 54.2% of activity compared to L-norleucine | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-norleucine + 2-oxoglutarate | Candida guilliermondii var. membranaefaciens | best amino donor, classical ping pong bi-bi mechanism | 2-oxohexanoate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida guilliermondii var. membranaefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate partial purification and several chromatographic steps, all steps at 4°C | Candida guilliermondii var. membranaefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26 | - |
pH 7.8, 35°C | Candida guilliermondii var. membranaefaciens |
Storage Stability | Organism |
---|---|
-25°C, stable in the presence of 0.01 mM pyridoxal 5'-phosphate and 0.01 mM 2-oxoglutarate | Candida guilliermondii var. membranaefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-N-methylornithine + 2-oxoglutarate | 10% of activity compared to L-norleucine | Candida guilliermondii var. membranaefaciens | 5-(methylamino)-2-oxopentanoic acid + L-glutamate | - |
? | |
6-N-acetyllysine + 2-oxoglutarate | 31.9% of activity compared to L-norleucine | Candida guilliermondii var. membranaefaciens | 6-(acetylamino)-2-oxohexanoic acid + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | 4.3% of activity compared to L-norleucine | Candida guilliermondii var. membranaefaciens | oxaloacetate + L-glutamate | - |
? | |
L-isoleucine + 2-oxoglutarate | 7.7% of activity compared to L-norleucine | Candida guilliermondii var. membranaefaciens | 3-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-leucine + 2-oxoglutarate | 54.2% of activity compared to L-norleucine | Candida guilliermondii var. membranaefaciens | 4-methyl-2-oxopentanoate + L-glutamate | - |
? | |
L-norleucine + 2-oxoglutarate | best amino donor, classical ping pong bi-bi mechanism | Candida guilliermondii var. membranaefaciens | 2-oxohexanoate + L-glutamate | - |
? | |
L-norvaline + 2-oxoglutarate | 10% of activity compared to L-norleucine | Candida guilliermondii var. membranaefaciens | 2-oxopentanoate + L-glutamate | - |
? | |
additional information | glycine, L-alanine, L-tyrosine, beta-alanine, epsilon-aminocaproate, delta-aminovalerate, L-arginine, L-lysine or L-ornithine are no substrates | Candida guilliermondii var. membranaefaciens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 51000, SDS-PAGE | Candida guilliermondii var. membranaefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Candida guilliermondii var. membranaefaciens |
55 | - |
- |
Candida guilliermondii var. membranaefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | reaction velocity increases linearly with increasing temperature at pH 7.8, above 55°C activity rapidly decreases, 2-oxoglutarate and to a lesser extent pyridoxal 5'-phosphate are effective protecting agents toward heating | Candida guilliermondii var. membranaefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
3 min, about 17% remaining activity, with addition of pyridoxal 5'-phosphate 70% remaining activity, with addition of 2-oxoglutarate 95% remaining activity | Candida guilliermondii var. membranaefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Candida guilliermondii var. membranaefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | absolute requirement, probably 1 mol pyridoxal 5'-phosphate binds to each subunit, apoenzyme is inactive and regains more than 92% of original enzymatic activity after addition of pyridoxal 5'-phosphate | Candida guilliermondii var. membranaefaciens |