Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-amino-8-oxononanoate | - |
Escherichia coli | |
7-Keto-8-aminopelargonic acid | competitive with adenosyl-L-methionine | Escherichia coli | |
adenosine | - |
Escherichia coli | |
S-Adenosyl-L-(2-hydroxy-4-methylthio)butyric acid | - |
Escherichia coli | |
S-adenosyl-L-ethionine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
S-adenosyl-L-methionine | pH 8.5, 37°C | Escherichia coli | |
0.001 | - |
7-amino-8-oxononanoate | pH 8.5, 37°C | Escherichia coli | |
0.0012 | - |
8-Amino-7-oxononanoate | pH 8.5, 37°C | Escherichia coli | |
0.021 | - |
pyridoxamine 5'-phosphate | pH 8.5, 37°C | Escherichia coli | |
0.032 | - |
pyridoxal 5'-phosphate | pH 8.5, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
2 * 47000, SDS-PAGE | Escherichia coli |
94000 | - |
gel filtration, sucrose density gradient sedimentation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
regulatory mutant | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
98 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, stable for 1 year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7,8-diketopelargonic acid | - |
Escherichia coli | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | - |
? | |
S-adenosyl-L-methionine + 7-amino-8-oxononanoate | - |
Escherichia coli | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | S-adenosyl-4-methylthio-2-oxobutanoate may be decomposed nonenzymatically to form 5'-methylthioadenosine and 2-oxo-3-butenoic acid | ? | |
S-adenosyl-L-methionine + 8-amino-7-oxononanoate | synonym: 7-keto-8-aminopelargonic acid, KAPA | Escherichia coli | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | S-adenosyl-4-methylthio-2-oxobutanoate may be decomposed nonenzymatically to form 5'-methylthioadenosine and 2-oxo-3-butenoic acid | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 47000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.283 | - |
8-Amino-7-oxononanoate | pH 8.5, 37°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
7-Keto-8-aminopelargonic acid | pH 8.5, 37°C | Escherichia coli |