Literature summary for 2.6.1.57 extracted from

Matsui, I.; Matsui, E.; Sakai, Y.; Kikuchi, H.; Kawarabayasi, Y.; Ura, H.; Kawaguchi, S.I.; Kuramitsu, S.; Harata, K.
The molecular structure of hyperthermostable aromatic aminotransferase with novel substrate specificity from Pyrococcus horikoshii (2000), J. Biol. Chem., 275, 4871-4879.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion, an equi-volume of 3 M 1,6-hexanediol solution at pH 7.5, 100 mM HEPES buffer, containing 10 mM MgCl2 is added to a protein solution containing 1.6% AeATPh and 0.02 mM pyridoxal 5'-phosphate, a droplet of the solution is equilibrated with 1 ml of 3 M 1,6-hexane-di-ol solution, crystals are grown at room temperature for 1 week, crystal structure of the native enzyme 2.1 a resolution, heavy atom derivatives diffract to 3.0 A resolution	Pyrococcus horikoshii

Crystallization (Comment)

Organism

hanging drop vapor diffusion, an equi-volume of 3 M 1,6-hexanediol solution at pH 7.5, 100 mM HEPES buffer, containing 10 mM MgCl2 is added to a protein solution containing 1.6% AeATPh and 0.02 mM pyridoxal 5'-phosphate, a droplet of the solution is equilibrated with 1 ml of 3 M 1,6-hexane-di-ol solution, crystals are grown at room temperature for 1 week, crystal structure of the native enzyme 2.1 a resolution, heavy atom derivatives diffract to 3.0 A resolution

Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	2 * 42000, recombinant enzyme, SDS-PAGE	Pyrococcus horikoshii
56000	-	recombinant enzyme, gel filtration	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, 80°C, HiTrap Q, HiLoad superdex 200	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-phenylalanine + 2-oxoglutarate	-	Pyrococcus horikoshii	phenylpyruvate + L-glutamate	-	?
L-tryptophan + 2-oxoglutarate	-	Pyrococcus horikoshii	3-indole-2-oxopropanoate + L-glutamate	-	?
L-tyrosine + 2-oxoglutarate	-	Pyrococcus horikoshii	p-hydroxyphenylpyruvate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 42000, recombinant enzyme, SDS-PAGE	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
ArATPh	-	Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	-	Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
95	-	no loss of activity after 30 min at pH 6.5	Pyrococcus horikoshii
110	-	half-life at pH 6.5: 30 min	Pyrococcus horikoshii

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	11	no loss of activity after 24 h at 25°C	Pyrococcus horikoshii

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Pyrococcus horikoshii