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Literature summary for 2.6.1.57 extracted from

  • Xing, R.; Whitman, W.B.
    Characterization of amino acid aminotransferases of Methanococcus aeolicus (1974), J. Bacteriol., 174, 541-548.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
cysteine
-
Methanococcus aeolicus
L-cysteine 10 mM Methanococcus aeolicus
methionine 10 mM, inhibits activity of isoenzyme ArAT-I with 1 mM tyrosine by 23%, isoenzyme ArAT-II is not affected Methanococcus aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase II Methanococcus aeolicus
0.4
-
L-phenylalanine pH 7.5, 37°C, aromatic aminotransferase I Methanococcus aeolicus
0.4
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tyrosine, aromatic aminotransferase I Methanococcus aeolicus
0.6
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase I Methanococcus aeolicus
0.6
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-phenylalanine, aromatic aminotransferase II Methanococcus aeolicus
1
-
L-tyrosine pH 7.5, 37°C, aromatic aminotransferase I Methanococcus aeolicus
1
-
L-tryptophan pH 7.5, 37°C, aromatic aminotransferase I Methanococcus aeolicus
1.2
-
L-phenylalanine pH 7.5, 37°C, aromatic aminotransferase II Methanococcus aeolicus
1.8
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase I Methanococcus aeolicus
1.9
-
L-tryptophan pH 7.5, 37°C, aromatic aminotransferase II Methanococcus aeolicus
2.1
-
L-tyrosine pH 7.5, 37°C, aromatic aminotransferase II Methanococcus aeolicus
4.6
-
2-oxoglutarate pH 7.5, 37°C, cosubstrate: L-tryptophan, aromatic aminotransferase II Methanococcus aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
ArAT-II, gel filtration Methanococcus aeolicus
150000
-
ArAT-I, gel filtration Methanococcus aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-phenylalanine + 2-oxoglutarate Methanococcus aeolicus
-
phenylpyruvate + L-glutamate
-
?
L-tryptophan + 2-oxoglutarate Methanococcus aeolicus
-
3-indole-2-oxopropanoate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate Methanococcus aeolicus
-
p-hydroxyphenylpyruvate + L-glutamate
-
?

Organism

Organism UniProt Comment Textmining
Methanococcus aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Methanococcus aeolicus
partial purification of enzyme forms ArAT-I and ArAT-II Methanococcus aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 2-oxoglutarate
-
Methanococcus aeolicus phenylpyruvate + L-glutamate
-
?
L-tryptophan + 2-oxoglutarate
-
Methanococcus aeolicus 3-indole-2-oxopropanoate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate
-
Methanococcus aeolicus p-hydroxyphenylpyruvate + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
ArAT-I
-
Methanococcus aeolicus
aromatic aminotransferase
-
Methanococcus aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanococcus aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Methanococcus aeolicus